{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:17:22Z","timestamp":1791580642751,"version":"4.3.4"},"reference-count":43,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Tianjin Medical University General Hospital \u201cNew Century Talents\u201d Cultivation Project","award":["XSJ-2021-02"],"award-info":[{"award-number":["XSJ-2021-02"]}]},{"name":"Tianjin Medical University General Hospital Young and Middle-Aged Scientific Research Cultivation Fund","award":["303078100501"],"award-info":[{"award-number":["303078100501"]}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82100352"],"award-info":[{"award-number":["82100352"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82202212"],"award-info":[{"award-number":["82202212"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Sepsis-induced cardiomyopathy, a life-threatening complication of sepsis, carries a significant mortality risk. Despite its clinical severity, the immunometabolic mechanisms driving its pathogenesis remain poorly understood. This study aimed to identify immunometabolism-associated genes in sepsis-induced cardiomyopathy and analyze immune infiltration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We integrated single-cell and bulk RNA sequencing data from septic cohorts, using a multistep bioinformatic approach involving machine learning and weighted gene co-expression network analysis to identify key immunometabolism-associated genes and explore their role in sepsis-induced cardiomyopathy. Key findings were validated using in vitro and in vivo experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We identified two distinct macrophage subsets (functional and senescent) and prioritized four hub genes, including ABLIM1, BCL9L, SMAD3, and DHRS3, which formed a robust prognostic model (area under the curve\u2009=\u20090.724). In vivo and in vitro validation confirmed significant downregulation of these hub genes in sepsis-induced cardiomyopathy. Immune infiltration analysis demonstrated significant increases in monocytes, M0\/M1 macrophages, and neutrophils in sepsis, with hub genes showing significant correlations with specific immune cell subsets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study identifies ABLIM1, BCL9L, SMAD3, and DHRS3 genes as pivotal biomarkers and potential therapeutic targets in sepsis-induced cardiomyopathy, linked to immune\u2013metabolic dysregulation and myocardial injury. These findings provide new insights for early diagnosis and precision immunotherapy of sepsis-induced cardiomyopathy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605251395584","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T04:12:45Z","timestamp":1763525565000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of immunometabolism-associated genes and immune infiltration in sepsis-induced cardiomyopathy using integrated bioinformatics and machine learning approaches"],"prefix":"10.1177","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6105-2774","authenticated-orcid":false,"given":"Chongzhe","family":"Pei","sequence":"first","affiliation":[{"name":"Tianjin Medical University General Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Tianjin Medical University General Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Tianjin Medical University General Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningning","family":"Song","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0287"},{"key":"e_1_3_7_3_2","first-page":"30","article-title":"Pathophysiology of sepsis-induced myocardial dysfunction","volume":"3","author":"Lv X","year":"2016","unstructured":"Lv X, Wang H. 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