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Cell types were annotated using Seurat. Differentially expressed gene analysis, pseudotime trajectory assessment, copy number variation analysis, and cell\u2013cell communication analyses were performed between high- and low-methylation groups. Validation was conducted using bulk RNA sequencing data from The Cancer Genome Atlas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Metastatic lymph nodes showed significantly lower RNA methylation scores than primary cancers, particularly in T cells and B cells. Low methylation was correlated with enhanced cell differentiation, increased malignancy in epithelial cells, and reduced immune cell communication. Monocytes exhibited opposite trends. Differentially expressed genes from methylation groups stratified The Cancer Genome Atlas patients into two clusters with distinct survival and immune infiltration profiles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>RNA methylation is suppressed during breast cancer metastasis, affecting cell differentiation, immune communication, and patient outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605251405638","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:20:51Z","timestamp":1765844451000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Single-cell analysis reveal cell-type-specific RNA methylation patterns in breast cancer"],"prefix":"10.1177","volume":"53","author":[{"given":"Shengtao","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Breast and Thyroid Surgery, Fujian Provincial Maternal and Child Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, First Affiliated Hospital of Fujian Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobing","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, Fujian Provincial Maternal and Child Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuju","family":"Han","sequence":"additional","affiliation":[{"name":"Zhejiang Chinese Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruilei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, Fujian Provincial Maternal and Child Hospital, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2019-4861","authenticated-orcid":false,"given":"Yong","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound Medicine, First Affiliated Hospital of Fujian Medical University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,12,15]]},"reference":[{"key":"e_1_3_10_2_2","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2019.20.7.2015"},{"key":"e_1_3_10_3_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.20.03399"},{"key":"e_1_3_10_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12282-022-01400-x"},{"key":"e_1_3_10_5_2","doi-asserted-by":"crossref","first-page":"e00722","DOI":"10.1002\/advs.202500722","article-title":"Paracrine orchestration of tumor microenvironment remodeling induced by GLO1 potentiates lymph node metastasis in breast cancer","volume":"12","author":"Xie J","year":"2025","unstructured":"Xie J, Liu W, Deng X, et\u00a0al. 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