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Cell viability and apoptosis were detected via Cell Counting Kit-8 and terminal deoxynucleotidyl transferase dUTP nick end labeling assays, respectively. Cellular morphology was assessed via transmission electron microscopy. Genetic changes were analyzed using transcriptome sequencing. Meanwhile, metandienone (0.1\u2009mg\u00b7g\n                      <jats:sup>\u22121<\/jats:sup>\n                      \u00b7d\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) was administered via gavage to mice for 14 days. Liver pathology was examined via hematoxylin and eosin, periodic acid\u2013Schiff, and transmission electron microscopy. Serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, albumin, total cholesterol, triglyceride, lactate dehydrogenase, and creatine kinase were measured.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Cell Counting Kit-8 assay confirmed that cell viability decreased to 88.01% after 24\u2009h of metandienone treatment. Terminal deoxynucleotidyl transferase dUTP nick end labeling assay showed that the apoptosis rate increased to 25.08%, compared with 4.94% in the NC group. RNA transcriptome data showed that the expression levels of 221 genes in the metandienone group altered significantly; these genes were closely related to liver metabolism, oxidative stress, inflammatory response, and immune regulation. Meanwhile, exposure to metandienone-induced liver injury was characterized by hepatocyte steatosis and inflammation, accompanied by significant increases in serum alanine aminotransferase and aspartate aminotransferase.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The abuse of anabolic hormones leads to liver function damage, and the mechanism may be related to inflammatory responses and metabolism.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605261448774","type":"journal-article","created":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T04:47:26Z","timestamp":1781326046000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Metandienone abuse induces hepatotoxicity via dysregulated inflammation and metabolic dysfunction"],"prefix":"10.1177","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6321-7897","authenticated-orcid":false,"given":"Yangyang","family":"Ye","sequence":"first","affiliation":[{"name":"Nanjing Police University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Nanjing University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuodong","family":"Chen","sequence":"additional","affiliation":[{"name":"Nanjing Police University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Xue","sequence":"additional","affiliation":[{"name":"Nanjing Police University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinzhong","family":"Xi","sequence":"additional","affiliation":[{"name":"Nanjing Police University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"key":"e_1_3_11_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3455"},{"key":"e_1_3_11_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2025.106801"},{"key":"e_1_3_11_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/00071668008416639"},{"key":"e_1_3_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2019.03.023"},{"key":"e_1_3_11_6_2","first-page":"148","article-title":"Determination of nine food-borne stimulant drug residues in pork, egg, and milk by ultra-performance liquid chromatography-tandem mass spectrometry]","volume":"40","author":"Liu X","year":"2022","unstructured":"Liu X, Zhao Y, Ma Y, et\u00a0al. 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