{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T04:52:30Z","timestamp":1781758350653,"version":"3.54.5"},"reference-count":28,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Lei Chen","award":["2017KA07"],"award-info":[{"award-number":["2017KA07"]}]},{"name":"Lei Chen","award":["2021xkj175"],"award-info":[{"award-number":["2021xkj175"]}]},{"name":"Lei Chen","award":["No. 2022AH050762"],"award-info":[{"award-number":["No. 2022AH050762"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Int Med Res"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study investigated whether metastasis-associated protein 1 (MTA1) acts as an RNA-binding protein to directly regulate gene expression and alternative splicing in prostate cancer, thereby exploring its dual transcriptional and post-transcriptional roles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>MTA1-stable-knockdown PC-3 cells were subjected to RNA sequencing and formaldehyde RNA immunoprecipitation sequencing to characterize the MTA1\u2013RNA interactome and its functional significance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MTA1 knockdown dysregulated 1248 genes and 2367 alternative splicing events. Differentially expressed genes were enriched in extracellular matrix remodeling, PI3K\u2013Akt signaling, and hypoxia-response pathways, whereas alternatively spliced genes were associated with spliceosome and RNA-processing pathways. Formaldehyde RNA immunoprecipitation sequencing confirmed MTA1 binding to GC-rich RNA motifs, with significant overlap between MTA1-bound targets and dysregulated genes or splicing events. Representative findings included MTA1 binding to the 3\u2032 untranslated region of COL6A1, resulting in its downregulation, and regulation of SNHG17 splicing, indicating direct roles in RNA stability and splicing regulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MTA1 functions as both an epigenetic modulator and an RNA-binding protein in prostate cancer, directly regulating RNA networks that promote tumor progression. These findings reveal novel RNA-mediated oncogenic mechanisms and highlight the therapeutic potential of MTA1 in prostate cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03000605261452954","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:19:26Z","timestamp":1781569166000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Metastasis-associated protein 1 rewires prostate cancer transcriptomes via direct RNA interactions governing gene expression and alternative splicing"],"prefix":"10.1177","volume":"54","author":[{"given":"Guanlin","family":"Qu","sequence":"first","affiliation":[{"name":"The Second Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9796-3928","authenticated-orcid":false,"given":"Fuhao","family":"Li","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enlai","family":"Li","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Xu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3258-695X","authenticated-orcid":false,"given":"Lei","family":"Chen","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"e_1_3_8_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2025.2470511"},{"key":"e_1_3_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.01.040"},{"key":"e_1_3_8_4_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.109593"},{"key":"e_1_3_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.02.012"},{"key":"e_1_3_8_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-022-00263-w"},{"key":"e_1_3_8_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-023-04579-4"},{"key":"e_1_3_8_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-06942-w"},{"key":"e_1_3_8_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2025.0228"},{"key":"e_1_3_8_10_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389203723666220705152713"},{"key":"e_1_3_8_11_2","doi-asserted-by":"publisher","DOI":"10.1200\/OP.24.00173"},{"key":"e_1_3_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2022.06.015"},{"key":"e_1_3_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2020.100728"},{"key":"e_1_3_8_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-022-09726-7"},{"key":"e_1_3_8_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-022-02241-4"},{"key":"e_1_3_8_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2023.1326148"},{"key":"e_1_3_8_17_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-20-0555"},{"key":"e_1_3_8_18_2","doi-asserted-by":"crossref","unstructured":"Venkatasubramanian M Schwartz L Ramachandra N et\u00a0al. Broad de-regulated U2AF1 splicing is prognostic and augments leukemic transformation via protein arginine methyltransferase activation. Epub ahead of print 8 February 2024. DOI: 10.1101\/2024.02.04.578798.","DOI":"10.1101\/2024.02.04.578798"},{"key":"e_1_3_8_19_2","doi-asserted-by":"crossref","unstructured":"Holmes ME Hertel KJ. Interdependent regulation of alternative splicing by SR and hnRNP proteins. Epub ahead of print 20 August 2024. DOI: 10.1101\/2024.08.19.608666.","DOI":"10.1101\/2024.08.19.608666"},{"key":"e_1_3_8_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/03007995.2024.2416985"},{"key":"e_1_3_8_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222111790"},{"key":"e_1_3_8_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16223766"},{"key":"e_1_3_8_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317"},{"key":"e_1_3_8_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1621"},{"key":"e_1_3_8_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_3_8_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr483"},{"key":"e_1_3_8_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1510"},{"key":"e_1_3_8_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2023.106427"},{"key":"e_1_3_8_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25073904"}],"container-title":["Journal of International Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03000605261452954","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03000605261452954","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03000605261452954","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T04:16:23Z","timestamp":1781756183000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03000605261452954"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":28,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1177\/03000605261452954"],"URL":"https:\/\/doi.org\/10.1177\/03000605261452954","relation":{},"ISSN":["0300-0605","1473-2300"],"issn-type":[{"value":"0300-0605","type":"print"},{"value":"1473-2300","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"article-number":"03000605261452954"}}