{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:29:11Z","timestamp":1790980151544,"version":"4.1.0"},"reference-count":28,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2004,9,1]],"date-time":"2004-09-01T00:00:00Z","timestamp":1093996800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tumori"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:sec>\n                    <jats:title>Aims and Background<\/jats:title>\n                    <jats:p>Cells and soluble mediators obtained from tumor effusions are useful in evaluating the tumor microenvironment. Our aim was to examine cytologically and to quantify the leukocyte infiltrate, nitric oxide, cytokines and tumor markers in the intracystic fluid from patients with a cystic adnexal mass, for a possible differentiation between benign and malignant findings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Study Design<\/jats:title>\n                    <jats:p>Sixty-six women who had their cystic fluids collected were prospectively divided into benign tumor (22, 33.3%), malignant tumor (10, 15.2%) or other gynecological alterations (34, 51.5%). Cytology, total and differential leukocyte counts were determined by light microscopy. Tumor markers, cytokines and nitric oxide were assayed in the supernatants using the Immutile system, ELISA and Griess reaction, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The sensitivity and specificity of the cytological analysis was 66.7% and 97.7%, respectively. The levels of CA 19.9, CA 15.3, \u03b1-fetoprotein, carcinoembryonic antigen, progesterone and \u03b2-HCG were significantly higher in the benign and\/or malignant group than in the other gynecological alterations. Also, the local concentrations of CA 15.3 and \u03b2-HCG were significantly higher in malignant than in benign tumors. In malignant tumors, increased leukocyte counts and higher concentrations of IL-6, IL-10 and nitric oxide were detected than in benign tumors or other gynecological alterations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In malignant tumors, the microenvironment could be differentiated from benign tumors or other gynecological alterations by cystic fluid analysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/030089160409000509","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T05:27:46Z","timestamp":1523942866000},"page":"491-497","source":"Crossref","is-referenced-by-count":20,"title":["Differential Tumor Microenvironment in Human Ovarian Cystic Tumors"],"prefix":"10.1177","volume":"90","author":[{"given":"Beatriz Martins Tavares","family":"Murta","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Faculty of Medicine of Tri\u00e2ngulo Mineiro, Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"De Queir\u00f3z Cunha","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Miranda","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Medicine of Tri\u00e2ngulo Mineiro, Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila Jorge","family":"Adad","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Medicine of Tri\u00e2ngulo Mineiro, Uberaba, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eddie Fernando Candido","family":"Murta","sequence":"additional","affiliation":[{"name":"Discipline 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