{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T19:06:38Z","timestamp":1781895998641,"version":"3.54.5"},"reference-count":45,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tumori"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:sec>\n                    <jats:title>Aims and background<\/jats:title>\n                    <jats:p>Cervical intraepithelial neoplasia (CIN) grade II-III is being diagnosed in younger women and, because of the reproductive age range for women and the habits associated with a modern lifestyle, is now affecting a broad age range. Surgical treatment for CIN has been associated with premature amenorrhea, low birth weight, and premature labor and birth. It is therefore imperative to develop clinical treatments for CIN, such as conservative treatment with interferons. The object of the present study was to evaluate the behavior of cytokines (IFN-\u03b3, IL-1\u03b2, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, TNF-\u03b1, TGF \u03b2) in the serum of patients with an initial diagnosis of CIN II-III.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Ten patients with CIN-CIN II (60%, n = 6) and CIN III (40%, n = 4), 23 to 51 years of age and who had not received any prior treatments, were evaluated. The patients were given 3 million\/UI (per cm\n                      <jats:sup>2<\/jats:sup>\n                      of colposcopic lesion) of human recombinant IFN-\u03b1 2b by intralesional administration (18 applications on alternate days). Before treatment, in the 6\n                      <jats:sup>th<\/jats:sup>\n                      , 12\n                      <jats:sup>th<\/jats:sup>\n                      , and 18\n                      <jats:sup>th<\/jats:sup>\n                      applications, blood was collected from the patients for cytokine analysis using ELISA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Half of the patients had a good pathologic response; the other half, all of whom were smokers, had therapeutic failure. The average concentration of IL-12 (pg\/ml) in the serum of patients who responded well to therapy was elevated from the 12\n                      <jats:sup>th<\/jats:sup>\n                      and 18\n                      <jats:sup>th<\/jats:sup>\n                      application of IFN-\u03b1 2b compared to patients who experienced therapeutic failure: 1804.0 \u00b1 1020 vs 391.2 \u00b1 722.3 and 1738.0 \u00b1 2426.0 vs 448.5 \u00b1 407.2, respectively, P &lt;0.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CIN II-III treated with intralesional IFN-\u03b1 2b achieved a good response in non-smoking patients and was associated with an increase in IL-12 serum levels.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/030089161109700507","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T04:21:18Z","timestamp":1519705278000},"page":"578-584","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Cytokine serum levels in patients with cervical intraepithelial neoplasia grade II-III treated with intralesional interferon-\u03b1 2b"],"prefix":"10.1177","volume":"97","author":[{"given":"Daniela Ribeiro","family":"Misson","sequence":"first","affiliation":[{"name":"Gynecology and Obstetrics, Federal University of the Tri\u00e2ngulo Mineiro, Uberaba, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas Reis","family":"Abdalla","sequence":"additional","affiliation":[{"name":"General Pathology Oncology Research Institute (Instituto de Pesquisa em Oncologia-IPON), Federal University of the Tri\u00e2ngulo Mineiro, Uberaba, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariana Melo","family":"Borges","sequence":"additional","affiliation":[{"name":"General Pathology Oncology Research Institute (Instituto de Pesquisa em Oncologia-IPON), Federal University of the Tri\u00e2ngulo Mineiro, Uberaba, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis Sakamoto","family":"Shimba","sequence":"additional","affiliation":[{"name":"Oncology Research Institute (IPON)\/Discipline of Gynecology and Obstetrics, Federal University of the Tri\u00e2ngulo Mineiro, Uberaba, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila Jorge","family":"Adad","sequence":"additional","affiliation":[{"name":"Discipline of Special Pathology, Federal University of the Tri\u00e2ngulo Mineiro, Uberaba, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia Antoniazi","family":"Michelin","sequence":"additional","affiliation":[{"name":"Oncology Research Institute (IPON)\/Discipline of Immunology, Federal University of the Tri\u00e2ngulo Mineiro, Uberaba, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eddie Fernando Candido","family":"Murta","sequence":"additional","affiliation":[{"name":"Oncology Research Institute (IPON)\/Discipline of Gynecology and Obstetrics, Federal University of the Tri\u00e2ngulo Mineiro, Uberaba, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,1,29]]},"reference":[{"key":"bibr1-030089161109700507","unstructured":"KumarV., AbbasA.K., FaustoN., MitchellR.N.: Robbins Basic Pathology (8th edn), pp 718\u2013721, Saunders Elsevier, 2007."},{"key":"bibr2-030089161109700507","unstructured":"World Health Organization: Human papillomavirus infection and cervical cancer, 2009. 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