{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:38:23Z","timestamp":1792715903874,"version":"4.5.0"},"reference-count":36,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Tumori"],"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>The present study aims to investigate the feasibility and therapeutic potential of intravesically administered interleukin-12 mRNA-loaded lipid nanoparticles (IL-12 mRNA LNPs) for treatment of orthotopic bladder cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Fluc-mRNA LNPs and IL-12 mRNA LNPs were prepared using microfluidic technology and characterized in terms of particle size, zeta potential, encapsulation efficiency, and stability. The\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      transfection efficiency of the Fluc-mRNA LNPs were assessed by bioluminescence imaging. The antitumor efficacy of IL-12 mRNA LNPs was valuated in orthotopic bladder cancer mice models which were established with Luc-MB49 cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Fluc-mRNA LNPs and IL-12 mRNA LNPs were successfully prepared via microfluidics, which had uniform particle size (101.6 nm and 104.4 nm), near-neutral surface charge (-3.39 mV and -8.34 mV), high encapsulation efficiency (89.01% and 98.87%), and good stability. Efficient intravesical transfection and robust protein expression were confirmed in mouse bladders using an\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      bioluminescence imaging. In orthotopic bladder cancer\u2013bearing mice, intravesical instillation of IL-12 mRNA LNPs significantly inhibited tumor growth.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Intravesical delivery of IL-12 mRNA LNPs represents an effective and promising strategy for local immunotherapy of bladder cancer. These findings provide experimental evidence supporting the potential clinical translation of mRNA-LNPs-based intravesical regimens for the treatment of bladder cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03008916261463130","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T05:20:56Z","timestamp":1784870456000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary study on intravesical instillation of IL-12 mRNA lipid nanoparticles for the treatment of bladder cancer"],"prefix":"10.1177","author":[{"given":"Bangjie","family":"Ouyang","sequence":"first","affiliation":[{"name":"School of Materials and Science and Engineering, East China University of Science and Technology, Shanghai, China"},{"name":"Interventional Medical Technology (Hangzhou) Company Limited, Hangzhou, Zhejiang Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaofeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Interventional Medical Technology (Hangzhou) Company Limited, Hangzhou, Zhejiang Province, China"},{"name":"School of Pharmacy, Anhui Medical University\u200c, Hefei, Anhui Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawen","family":"Li","sequence":"additional","affiliation":[{"name":"Interventional Medical Technology (Hangzhou) Company Limited, Hangzhou, Zhejiang Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanqiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Interventional Medical Technology (Hangzhou) Company Limited, Hangzhou, Zhejiang Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3661-6320","authenticated-orcid":false,"given":"Yuanyuan","family":"Shen","sequence":"additional","affiliation":[{"name":"Interventional Medical Technology (Hangzhou) Company Limited, Hangzhou, Zhejiang Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meidong","family":"Lang","sequence":"additional","affiliation":[{"name":"School of Materials and Science and Engineering, East China University of Science and Technology, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21871"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.09.001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.21037\/tau.2019.09.38"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2017.16"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2016.03.034"},{"key":"e_1_3_2_8_2","article-title":"Quadruple immunotherapy of Bacillus Calmette-Gu\u00e9rin, interferon, interleukin-2, and granulocyte-macrophage colony-stimulating factor as salvage therapy for non\u2013muscle-invasive bladder cancer","volume":"35","author":"Steinberg RL","year":"2017","unstructured":"Steinberg RL, Nepple KG, Velaer KN, et al. 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