{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T09:46:49Z","timestamp":1786787209264,"version":"build-2736575974"},"reference-count":34,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T00:00:00Z","timestamp":1370304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>Melanoma diagnosis in dogs can be challenging due to the variety of histological appearances of canine melanocytic neoplasms. Markers of malignancy are needed. Receptor for activated C-kinase 1 (RACK1) was found to characterize melanomas in other mammals. We investigated the value of RACK1 detection in the classification of 19 cutaneous and 5 mucosal melanocytic neoplasms in dogs. These tumors were categorized as melanocytomas or benign and melanomas or malignant after evaluation of their morphology, mitotic index, and Ki-67 growth fraction. Using immunofluorescence, we confirmed microphthalmia-associated transcription factor (MITF) as a marker of normal and transformed melanocytic cells in dog tissues. All control ( n = 10) and tumoral ( n = 24) samples stained positively for MITF (34\/34, 100%). Whereas RACK1 was not detected in healthy skin melanocytes, melanocytic lesions were all positive for RACK1 signal (24\/24, 100%). RACK1 cytoplasmic staining appeared with 2 distinct distribution patterns: strong, diffuse, and homogeneous or granular and heterogeneous. All melanoma samples (13\/13, 100%) stained homogeneously for RACK1. All melanocytomas (11\/11, 100%) stained heterogeneously for RACK1. Immunohistochemistry was less consistent than immunofluorescence for all labelings in melanocytic lesions, which were often very pigmented. Thus, the fluorescent RACK1-MITF labeling pattern helped to distinguish melanomas from melanocytomas. Furthermore, RACK1 labeling correlated with 2 of 11 morphological features linked to malignancy: cell and nuclear size. These results suggest that RACK1 may be used as a marker in dog melanomas.<\/jats:p>","DOI":"10.1177\/0300985813490754","type":"journal-article","created":{"date-parts":[[2013,6,5]],"date-time":"2013-06-05T01:54:10Z","timestamp":1370397250000},"page":"1083-1090","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":19,"title":["Canine Melanoma Diagnosis"],"prefix":"10.1177","volume":"50","author":[{"given":"C.","family":"Campagne","sequence":"first","affiliation":[{"name":"INRA, UMR955 de G\u00e9n\u00e9tique fonctionnelle et m\u00e9dicale, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"},{"name":"Universit\u00e9 Paris Est, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Jul\u00e9","sequence":"additional","affiliation":[{"name":"INRA, UMR955 de G\u00e9n\u00e9tique fonctionnelle et m\u00e9dicale, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"},{"name":"Universit\u00e9 Paris Est, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"},{"name":"Laboratoire Physiopathologie Orale et Mol\u00e9culaire, Centre de Recherche des Cordieliers, INSERM UMRS 872, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Alleaume","sequence":"additional","affiliation":[{"name":"Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Service d\u2019Anatomie pathologique, Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Bernex","sequence":"additional","affiliation":[{"name":"INRA, UMR955 de G\u00e9n\u00e9tique fonctionnelle et m\u00e9dicale, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"},{"name":"Universit\u00e9 Paris Est, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"},{"name":"Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Service d\u2019Anatomie pathologique, Maisons-Alfort, France"},{"name":"RHEM-R\u00e9seau d\u2019Histologie Exp\u00e9rimentale de Montpellier, Institut de Recherche en Canc\u00e9rologie de Montpellier, INSERM U896, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ezagal","sequence":"additional","affiliation":[{"name":"INRA, UMR955 de G\u00e9n\u00e9tique fonctionnelle et m\u00e9dicale, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"},{"name":"Universit\u00e9 Paris Est, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ch\u00e2teau-Joubert","sequence":"additional","affiliation":[{"name":"Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Service d\u2019Anatomie pathologique, Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Estrada","sequence":"additional","affiliation":[{"name":"Laboratoire IDEXX Alfort, Alfortville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Aubin-Houzelstein","sequence":"additional","affiliation":[{"name":"INRA, UMR955 de G\u00e9n\u00e9tique fonctionnelle et m\u00e9dicale, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"},{"name":"Universit\u00e9 Paris Est, Ecole Nationale V\u00e9t\u00e9rinaire d\u2019Alfort, Maisons-Alfort, France"},{"name":"Institut Pasteur, Unit\u00e9 de G\u00e9n\u00e9tique Fonctionnelle de la Souris; CNRS URA 2578, D\u00e9partement de Biologie du D\u00e9veloppement, USC INRA, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-J.","family":"Panthier","sequence":"additional","affiliation":[{"name":"INRA, UMR955 de G\u00e9n\u00e9tique fonctionnelle et m\u00e9dicale, Ecole Nationale 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