{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:39:16Z","timestamp":1792701556440,"version":"4.5.0"},"reference-count":25,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2016,8,20]],"date-time":"2016-08-20T00:00:00Z","timestamp":1471651200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Using transmission electron microscopy (TEM), the presented work expands on the ultrastructural findings of an earlier report on \u201csyncytial hepatitis,\u201d a novel disease of tilapia (SHT). Briefly, TEM confirmed the presence of an orthomyxovirus-like virus within the diseased hepatocytes but not within the endothelium. This was supported by observing extracellular and intracellular (mostly intraendosomal), 60\u2013100 nm round virions with a trilaminar capsid containing up to 7 electron-dense aggregates. Other patterns noted included enveloped or filamentous virions and virion-containing cytoplasmic membrane folds, suggestive of endocytosis. Patterns atypical for orthymyxovirus included the formation of syncytia and the presence of virions within the perinuclear cisternae (suspected to be the Golgi apparatus). The ultrastructural morphology of SHT-associated virions is similar to that previously reported for tilapia lake virus (TiLV). A genetic homology was investigated using the available reverse transcriptase polymerase chain reaction (RT-PCR) probes for TiLV and comparing clinically sick with clinically normal fish and negative controls. By RT-PCR analysis, viral nucleic acid was detected only in diseased fish. Taken together, these findings strongly suggest that a virus is causally associated with SHT, that this virus shares ultrastructural features with orthomyxoviruses, and it presents with partial genetic homology with TiLV (190 nucleotides).<\/jats:p>","DOI":"10.1177\/0300985816658100","type":"journal-article","created":{"date-parts":[[2016,8,10]],"date-time":"2016-08-10T22:28:59Z","timestamp":1470868139000},"page":"164-170","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":103,"title":["Syncytial Hepatitis of Tilapia (\n                    <i>Oreochromis niloticus<\/i>\n                    L.) is Associated With Orthomyxovirus-Like Virions in Hepatocytes"],"prefix":"10.1177","volume":"54","author":[{"given":"J.","family":"del-Pozo","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Mishra","sequence":"additional","affiliation":[{"name":"Columbia University, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Kabuusu","sequence":"additional","affiliation":[{"name":"St George\u2019s University, St Georges, Grenada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Cheetham","sequence":"additional","affiliation":[{"name":"St George\u2019s University, St Georges, Grenada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Eldar","sequence":"additional","affiliation":[{"name":"The Kimron Veterinary Institute, Bet Dagan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Bacharach","sequence":"additional","affiliation":[{"name":"Tel Aviv University, Tel Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. I.","family":"Lipkin","sequence":"additional","affiliation":[{"name":"Columbia University, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. W.","family":"Ferguson","sequence":"additional","affiliation":[{"name":"St George\u2019s University, St Georges, Grenada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,8,20]]},"reference":[{"key":"bibr1-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00431-16"},{"key":"bibr2-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2009.01037.x"},{"key":"bibr3-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-014-2235-8"},{"key":"bibr4-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.1998.00071.x"},{"key":"bibr5-0300985816658100","first-page":"356","volume-title":"Ultrastructural Pathology","author":"Cheville NF","year":"2009"},{"key":"bibr6-0300985816658100","first-page":"358","volume-title":"Ultrastructural Pathology","author":"Cheville NF","year":"2009"},{"key":"bibr7-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.1.218-227.2000"},{"key":"bibr8-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00827-14"},{"key":"bibr9-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12142"},{"key":"bibr10-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1965.tb14295.x"},{"key":"bibr11-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1139\/f85-165"},{"key":"bibr12-0300985816658100","doi-asserted-by":"publisher","DOI":"10.3354\/dao047087"},{"key":"bibr13-0300985816658100","doi-asserted-by":"publisher","DOI":"10.3354\/dao043081"},{"key":"bibr14-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1079\/AHR200461"},{"key":"bibr15-0300985816658100","doi-asserted-by":"publisher","DOI":"10.3354\/dao029099"},{"key":"bibr16-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0832269100"},{"key":"bibr17-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.05.002"},{"key":"bibr18-0300985816658100","doi-asserted-by":"publisher","DOI":"10.3354\/dao035145"},{"key":"bibr19-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.07.020"},{"key":"bibr20-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1354\/vp.42-3-338"},{"key":"bibr21-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.1974.tb05107.x"},{"key":"bibr22-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2005.00642.x"},{"key":"bibr23-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.01.001"},{"key":"bibr24-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1177\/030098589503200503"},{"key":"bibr25-0300985816658100","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-10-5"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300985816658100","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0300985816658100","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300985816658100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:57:13Z","timestamp":1777661833000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0300985816658100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,20]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1177\/0300985816658100"],"URL":"https:\/\/doi.org\/10.1177\/0300985816658100","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,20]]}}}