{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:12:58Z","timestamp":1784542378310,"version":"3.55.0"},"reference-count":21,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2018,6,20]],"date-time":"2018-06-20T00:00:00Z","timestamp":1529452800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>Hypophysectomy specimens from 16 dogs with pituitary adenoma were evaluated with periodic acid-Schiff (PAS), reticulin, and immunohistochemistry for adrenocorticotrophic hormone (ACTH), melanocyte stimulating hormone (MSH), growth hormone (GH), and Ki-67. The reticulin network was obliterated in all adenomas. One adenoma expressed ACTH and GH. Eight corticotroph adenomas were basophilic to chromophobic, and PAS- and ACTH-positive. Seven melanotroph adenomas were distinguished from corticotroph adenomas by expression of MSH. Pituitary-dependent hypercortisolism was diagnosed in 5 of 8 dogs with corticotroph and 4 of 7 with melanotroph adenoma. Pituitary height\/brain area (P\/B) ratio was elevated in all dogs. Previous canine hypophysectomy studies suggested that melanotroph adenomas were larger and carried a worse prognosis than corticotroph adenomas; however, in this study, corticotroph adenomas in comparison to melanotroph adenomas were larger (median P\/B ratio: 1.06 versus 0.76), more proliferative (median Ki-67 index: 9.47% versus 1.99%), and associated with shorter survival (median: 300 versus 793 days). Recommended immunohistochemistry for PAS-positive pituitary adenomas includes ACTH and MSH to distinguish corticotrophs from melanotrophs and Ki-67 for proliferation index.<\/jats:p>","DOI":"10.1177\/0300985818784160","type":"journal-article","created":{"date-parts":[[2018,6,20]],"date-time":"2018-06-20T22:57:56Z","timestamp":1529535476000},"page":"889-895","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Immunohistochemical Evaluation of Canine Pituitary Adenomas Obtained by Transsphenoidal Hypophysectomy"],"prefix":"10.1177","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1620-9303","authenticated-orcid":false,"given":"Margaret A.","family":"Miller","sequence":"first","affiliation":[{"name":"Indiana Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN, USA"},{"name":"Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina Jo","family":"Owen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences and Veterinary Teaching Hospital, Washington State University, Pullman, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Bruyette","sequence":"additional","affiliation":[{"name":"Anivive Life Sciences, Long Beach, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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