{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:50:49Z","timestamp":1779209449987,"version":"3.51.4"},"reference-count":9,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T00:00:00Z","timestamp":1582243200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100015501","name":"Exxon Valdez Oil Spill Trustee Council","doi-asserted-by":"crossref","award":["Project #19120111-E"],"award-info":[{"award-number":["Project #19120111-E"]}],"id":[{"id":"10.13039\/100015501","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:p>Over a 3-year-period, 17 wild-caught opaleye ( Girella nigricans) housed in a public display aquarium were found dead without premonitory signs. Grossly, 4 animals had pinpoint brown or black foci on coelomic adipose tissue. Histologically, liver, spleen, heart, and posterior kidney had mesomycetozoan granulomas in all cases; other organs were less commonly infected. Four opaleye had goiter; additional substantial lesions were not identified. Granulomas surrounded melanized debris, leukocytes, and mesomycetozoa represented by folded membranes (collapsed schizont walls), intact schizonts (50- to &gt;200 \u00b5m in diameter with a multilaminate membrane), plasmodia (budding from schizonts or free in tissue), or rarely germinal tubes (budding from schizonts). Ichthyophonus was grown from fresh tissues in tissue explant broth cultures of the heart, liver, and\/or spleen. Polymerase chain reaction using 18S ribosomal DNA primers amplified a 1730-bp region, and the DNA sequence was most similar to Ichthyophonus hoferi, which is often associated with freshwater aquaculture fish.<\/jats:p>","DOI":"10.1177\/0300985819900015","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T04:56:46Z","timestamp":1582261006000},"page":"316-320","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Ichthyophonus sp.<\/i>\n                    Infection in Opaleye (\n                    <i>Girella nigricans<\/i>\n                    )"],"prefix":"10.1177","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8510-1051","authenticated-orcid":false,"given":"Elise E. 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Ichthyophonus disease (ichthyophoniasis). In: FHS Blue Book: Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens. American Fisheries Society-Fish Health Section; 2016: https:\/\/units.fisheries.org\/fhs\/fish-health-section-blue-book-2016\/section-1-diagnostic\/. 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