{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T14:31:24Z","timestamp":1793025084020,"version":"4.5.0"},"reference-count":63,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100004486","name":"Fondazione Cassa di Risparmio di Perugia","doi-asserted-by":"crossref","award":["2013.0251.021"],"award-info":[{"award-number":["2013.0251.021"]}],"id":[{"id":"10.13039\/501100004486","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:p>E-cadherin, a glycoprotein involved in cell-cell adhesion, has a pivotal role in epithelial-mesenchymal transition, a process through which neoplastic epithelial cells develop an invasive phenotype. In human cutaneous melanomas, decreased E-cadherin expression is associated with shorter survival and increased Breslow thickness, whereas in the dog its role is poorly understood. Tumor thickness and modified Clark level were recently proposed as useful features to assess canine melanocytic tumors, but no studies investigated their association with E-cadherin expression. We performed immunohistochemistry on 77 formalin-fixed, paraffin-embedded primary canine melanocytic tumors. A 3-tier and a 2-tier classification system for assessing E-cadherin expression were tested, with the latter being more informative for the assessment of canine melanocytic tumors. E-cadherin expression was lower in cutaneous melanomas than melanocytomas, as well as in amelanotic tumors compared to pigmented tumors. In amelanotic melanomas, absent E-cadherin expression was associated with an unfavorable outcome, suggesting a potential use of this marker in defining the prognosis of amelanotic melanomas. E-cadherin expression was lower in tumors with greater tumor thickness and modified Clark level \u2265IV, suggesting its possible utility in identifying the most invasive tumors. The expression of E-cadherin in oral melanomas was heterogeneous, but was associated with pigmentation and clinical outcome; thus, E-cadherin evaluation could be advantageous to detect the most aggressive neoplasms. However, cutaneous melanomas without E-cadherin expression frequently had a favorable clinical outcome. Hence, its importance as prognostic factor should be carefully considered depending on the tumor origin.<\/jats:p>","DOI":"10.1177\/0300985820934385","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T06:22:11Z","timestamp":1592979731000},"page":"608-619","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["E-Cadherin Expression in Canine Melanocytic Tumors: Histological, Immunohistochemical, and Survival Analysis"],"prefix":"10.1177","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2366-1210","authenticated-orcid":false,"given":"Serenella","family":"Silvestri","sequence":"first","affiliation":[{"name":"University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2882-6739","authenticated-orcid":false,"given":"Ilaria","family":"Porcellato","sequence":"additional","affiliation":[{"name":"University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Mechelli","sequence":"additional","affiliation":[{"name":"University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8500-7970","authenticated-orcid":false,"given":"Laura","family":"Menchetti","sequence":"additional","affiliation":[{"name":"University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selina","family":"Iussich","sequence":"additional","affiliation":[{"name":"University of Torino, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaella","family":"De Maria","sequence":"additional","affiliation":[{"name":"University of Torino, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Sforna","sequence":"additional","affiliation":[{"name":"University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8942-6048","authenticated-orcid":false,"given":"Laura","family":"Bongiovanni","sequence":"additional","affiliation":[{"name":"University of Teramo, Teramo, Italy"},{"name":"Present address: Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4812-3216","authenticated-orcid":false,"given":"Chiara","family":"Brachelente","sequence":"additional","affiliation":[{"name":"University of Perugia, Perugia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,6,24]]},"reference":[{"key":"bibr1-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3481"},{"key":"bibr2-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2015.09.022"},{"issue":"3","key":"bibr3-0300985820934385","first-page":"187","volume":"28","author":"Bonitsis N","year":"2006","journal-title":"Exp Oncol"},{"key":"bibr4-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000138"},{"key":"bibr5-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3265"},{"key":"bibr6-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2013.08.018"},{"issue":"3","key":"bibr7-0300985820934385","first-page":"705","volume":"29","author":"Clark WJ","year":"1969","journal-title":"Cancer Res"},{"key":"bibr8-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.492"},{"issue":"3","key":"bibr9-0300985820934385","first-page":"449","volume":"53","author":"Fernandez-Flores A","year":"2012","journal-title":"Rom J Morphol"},{"key":"bibr10-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2015.03.001"},{"key":"bibr11-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1002\/9780470376928.ch2"},{"key":"bibr12-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985812457792"},{"key":"bibr13-0300985820934385","doi-asserted-by":"crossref","unstructured":"Han JI, Na KJ. Wnt\/\u03b2-catenin signaling pathway in canine skin melanoma and a possibility as a cancer model for human skin melanoma. In: Murph M, ed. Melanoma in the Clinic\u2014Diagnosis, Management and Complications of Malignancy. IntechOpen. 2011. Accessed May 30, 2020. https:\/\/www.intechopen.com\/books\/melanoma-in-the-clinic-diagnosis-management-and-complications-of-malignancy\/wnt-catenin-signaling-pathway-in-canine-skin-melanoma-and-a-possibility-as-a-cancer-model-for-human-","DOI":"10.5772\/21221"},{"key":"bibr14-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2012.10824.x"},{"key":"bibr15-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-2491"},{"key":"bibr16-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65023-7"},{"key":"bibr17-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2015.03.008"},{"key":"bibr18-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1590\/S1676-24442010000200007"},{"key":"bibr19-0300985820934385","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2013.00097"},{"key":"bibr20-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2018.03.004"},{"key":"bibr21-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-07-2729"},{"key":"bibr22-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1046\/j.0303-6987.2004.0106.x"},{"key":"bibr23-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.10.020"},{"issue":"8","key":"bibr24-0300985820934385","first-page":"393","volume":"163","author":"Lacroux C","year":"2012","journal-title":"Rev Med Vet (Toulouse)"},{"key":"bibr25-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12426"},{"issue":"9","key":"bibr26-0300985820934385","first-page":"981","volume":"59","author":"Laver T","year":"2018","journal-title":"Can Vet J"},{"issue":"9","key":"bibr27-0300985820934385","first-page":"3819","volume":"61","author":"Li G","year":"2001","journal-title":"Cancer Res"},{"key":"bibr28-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.061598"},{"key":"bibr29-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2005.10.004"},{"key":"bibr30-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985814547391"},{"key":"bibr31-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985815593349"},{"key":"bibr32-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(02)00041-3"},{"key":"bibr33-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2015.12.030"},{"key":"bibr34-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.25"},{"key":"bibr35-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2017.07.028"},{"key":"bibr36-0300985820934385","doi-asserted-by":"publisher","DOI":"10.4103\/abr.abr_146_18"},{"key":"bibr37-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1186\/s13620-016-0068-3"},{"key":"bibr38-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201008173"},{"key":"bibr39-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2017.09.009"},{"key":"bibr40-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1007\/s10577-014-9444-6"},{"key":"bibr41-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985818808530"},{"key":"bibr42-0300985820934385","unstructured":"R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing; 2016. Accessed May 30, 2020. https:\/\/www.r-project.org\/"},{"key":"bibr43-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810382095"},{"key":"bibr44-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985815620844"},{"key":"bibr45-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-3189"},{"key":"bibr46-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199812)186:4<340::AID-PATH182>3.0.CO;2-K"},{"key":"bibr47-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2017.03.005"},{"key":"bibr48-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-008-9179-y"},{"key":"bibr49-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31829d7f35"},{"key":"bibr50-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985818798094"},{"key":"bibr51-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13395"},{"key":"bibr52-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-159046"},{"key":"bibr53-0300985820934385","doi-asserted-by":"publisher","DOI":"10.2460\/javma.253.1.84"},{"key":"bibr54-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810390717"},{"key":"bibr55-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e3181f44abc"},{"key":"bibr56-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1354\/vp.43-2-136"},{"key":"bibr57-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.08246.x"},{"key":"bibr58-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1002\/path.4632"},{"key":"bibr59-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8281-1"},{"issue":"6","key":"bibr60-0300985820934385","first-page":"6545","volume":"18","author":"Varallo GR","year":"2019","journal-title":"Oncol Lett"},{"key":"bibr61-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810377187"},{"key":"bibr62-0300985820934385","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.000455"},{"key":"bibr63-0300985820934385","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2014.00367"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300985820934385","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0300985820934385","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0300985820934385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T19:00:52Z","timestamp":1777662052000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0300985820934385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,24]]},"references-count":63,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1177\/0300985820934385"],"URL":"https:\/\/doi.org\/10.1177\/0300985820934385","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,24]]}}}