{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:16:27Z","timestamp":1793420187644,"version":"4.5.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Ministry of Education","award":["2017R1D1A1B03032057"],"award-info":[{"award-number":["2017R1D1A1B03032057"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>Epidermal growth factor receptor (EGFR) is overexpressed in many human colorectal cancers and anti-EGFR agents are employed as immunotherapies. However, KRAS, EGFR, and BRAF gene mutations can influence the activity of the anti-EGFR agents. We evaluated EGFR expression at protein and mRNA levels in canine intestinal adenocarcinomas using immunohistochemistry (IHC) and RNA in situ hybridization (RNA-ISH). We also investigated the mutation status of EGFR, KRAS, and BRAF to aid the development of anti-EGFR agents for canine intestinal adenocarcinoma. EGFR expression was highest in adenocarcinoma, followed by intramucosal neoplasia (adenoma and in situ carcinoma), and nonneoplastic canine intestinal tissue, at both protein ( P = .000) and mRNA ( P = .005) levels. The EGFR, KRAS, and BRAF genes showed wild-type sequences at the mutation hot spots in all 13 specimens. Thus, EGFR might serve as a promising diagnostic marker in canine intestinal adenocarcinoma, and further studies would be needed to develop EGFR-targeted anticancer therapies.<\/jats:p>","DOI":"10.1177\/03009858211009778","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T03:10:15Z","timestamp":1619752215000},"page":"674-682","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["EGFR Overexpression and Sequence Analysis of KRAS, BRAF, and EGFR Mutation Hot Spots in Canine Intestinal Adenocarcinoma"],"prefix":"10.1177","volume":"58","author":[{"given":"Seung-Hee","family":"Cho","sequence":"first","affiliation":[{"name":"Konkuk University, Gwangjin-gu, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byung-Joon","family":"Seung","sequence":"additional","affiliation":[{"name":"Konkuk University, Gwangjin-gu, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1662-2651","authenticated-orcid":false,"given":"Soo-Hyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Konkuk University, Gwangjin-gu, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Kyung","family":"Bae","sequence":"additional","affiliation":[{"name":"Konkuk University, Gwangjin-gu, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha-Young","family":"Lim","sequence":"additional","affiliation":[{"name":"Konkuk University, Gwangjin-gu, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4496-7582","authenticated-orcid":false,"given":"Jung-Hyang","family":"Sur","sequence":"additional","affiliation":[{"name":"Konkuk University, Gwangjin-gu, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,4,30]]},"reference":[{"key":"bibr1-03009858211009778","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"bibr2-03009858211009778","doi-asserted-by":"publisher","DOI":"10.1016\/j.biolcel.2004.05.002"},{"key":"bibr3-03009858211009778","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200412303512724"},{"key":"bibr4-03009858211009778","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(01)00233-7"},{"key":"bibr5-03009858211009778","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2019.12.008"},{"key":"bibr6-03009858211009778","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.08.037"},{"key":"bibr7-03009858211009778","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2075"},{"key":"bibr8-03009858211009778","doi-asserted-by":"publisher","DOI":"10.1038\/ng0393-266"},{"key":"bibr9-03009858211009778","volume-title":"World Health Organization Classification of Tumours: Pathology and Genetics of Tumours of the Digestive System. 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