{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:30:58Z","timestamp":1777919458180,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.sagepub.com\/licence-information-for-chorus"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K15660"],"award-info":[{"award-number":["20K15660"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009035","name":"Program on Open Innovation Platform with Enterprises, Research Institute and Academia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009035","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>Keratinic primary localized cutaneous amyloidosis is a disease in humans; however, no similar condition has been reported in animals. This study aimed to investigate cutaneous keratinic amyloid deposition in dogs and elucidate its etiology. Canine hair follicle tumor tissues were histopathologically analyzed. Immunohistochemistry and mass spectrometry\u2013based proteomic analyses were performed to identify precursor protein candidates. Structural prediction and in vitro fibrillization analyses were conducted to determine the amyloidogenic region and gene sequencing analysis was performed to assess mutations. Of the 266 samples, 16 had amyloid deposition. Amyloid deposits were found in the stroma of tumors and in the margins of keratin debris and around normal hair follicles. Cytokeratin 5 (CK5) was identified as a precursor protein candidate. C-terminal truncation of CK5 was observed in amyloid deposits, and the truncation sites varied depending on the deposition pattern. There was a significantly higher incidence of amyloid deposition in Shiba dogs, and CK5 amino acid polymorphisms were identified in these dogs. A part of the C-terminal region of both canine and human CK5 exhibited highly amyloidogenic properties in vitro. This study revealed the existence of cutaneous keratinic amyloid deposition in animals and identified CK5 as an amyloid precursor protein, providing novel insights into understanding the etiology of cutaneous amyloidosis.<\/jats:p>","DOI":"10.1177\/03009858221128924","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T09:52:56Z","timestamp":1665481976000},"page":"60-68","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Keratinic amyloid deposition in canine hair follicle tumors"],"prefix":"10.1177","volume":"60","author":[{"given":"Kyoko","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Tokyo University of Agriculture and Technology, Fuchu-shi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susumu","family":"Iwaide","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology, Fuchu-shi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Sakai","sequence":"additional","affiliation":[{"name":"Gifu University, Gifu-shi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9125-7001","authenticated-orcid":false,"given":"Fuyuki","family":"Kametani","sequence":"additional","affiliation":[{"name":"Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-7842","authenticated-orcid":false,"given":"Tomoaki","family":"Murakami","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology, Fuchu-shi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,10,11]]},"reference":[{"key":"bibr1-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1080\/13506120500032543"},{"key":"bibr2-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.17630"},{"key":"bibr3-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2018.03.060"},{"key":"bibr4-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-004-0474-3"},{"key":"bibr5-03009858221128924","doi-asserted-by":"publisher","DOI":"10.18203\/issn.2455-4529.IntJResDermatol20204563"},{"key":"bibr6-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1080\/13506129.2020.1811962"},{"key":"bibr7-03009858221128924","volume":"11","author":"Cohen PR.","year":"2019","journal-title":"Cureus"},{"key":"bibr8-03009858221128924","first-page":"473","volume":"116","author":"Eto H","year":"1984","journal-title":"Am J Pathol"},{"key":"bibr9-03009858221128924","volume":"10","author":"Fang S","year":"2015","journal-title":"PLoS ONE"},{"key":"bibr10-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1111\/ane.13035"},{"key":"bibr11-03009858221128924","first-page":"89","volume-title":"Tumors in Domestic Animals","volume":"2016","author":"Goldschmidt MH"},{"key":"bibr12-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1293\/tox.2017-0044"},{"key":"bibr13-03009858221128924","doi-asserted-by":"publisher","DOI":"10.3109\/13506120009146439"},{"key":"bibr14-03009858221128924","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.581936"},{"key":"bibr15-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12531130"},{"key":"bibr16-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.63.1"},{"key":"bibr17-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12581609"},{"key":"bibr18-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz268"},{"key":"bibr19-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpdhp.2019.12.004"},{"key":"bibr20-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16182"},{"key":"bibr21-03009858221128924","volume":"11","author":"Mehrotra K","year":"2017","journal-title":"J Clin Diagnostic Res"},{"key":"bibr22-03009858221128924","first-page":"469","volume-title":"Amyloid and Eelated Disorders","volume":"2015","author":"Mereuta OM"},{"key":"bibr23-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2020.03.007"},{"key":"bibr24-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1684\/ejd.2010.0842"},{"key":"bibr25-03009858221128924","first-page":"667","volume-title":"Muller and Kirk\u2019s Small Animal Dermatology","volume":"2001","author":"Scott DW"},{"key":"bibr26-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-003-0464-5"},{"key":"bibr27-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku399"},{"key":"bibr28-03009858221128924","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/427296"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858221128924","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03009858221128924","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858221128924","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858221128924","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T19:03:37Z","timestamp":1777662217000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03009858221128924"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,11]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1177\/03009858221128924"],"URL":"https:\/\/doi.org\/10.1177\/03009858221128924","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,11]]}}}