{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T15:58:27Z","timestamp":1781020707574,"version":"3.54.1"},"reference-count":27,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:00:00Z","timestamp":1705017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:p>\n                    Canine splenic hemangiosarcoma has a high metastatic rate and short survival time. Currently, the main prognostic parameters are tumor stage and therapy, while data on histologic parameters, such as grade and Ki-67 expression, are scarce. The aims of this study were to compare two methods of assessment of Ki-67, verify their prognostic impact, and define a threshold value based on survival. Thirty-one cases of histologically diagnosed canine splenic hemangiosarcoma, which were treated with splenectomy and had full staging and follow-up information, were collected. Three were stage I, 17 stage II, and 11 stage III. The mean mitotic count (MC) was 23.9 (standard deviation [SD]: 22.1) and the median was 15 (range, 1\u201393). Immunohistochemistry for Ki-67 was performed, the Ki-67 labeling index (Ki-67LI) was assessed as a percentage of positive neoplastic nuclei per \u2265500 cell, and the Ki-67 count (KI-67C) was defined as the average number of positive nuclei using a 1 cm\n                    <jats:sup>2<\/jats:sup>\n                    optical grid performed in 5, 40\u00d7 fields. The mean Ki-67LI and Ki-67C were 56.4% (SD: 38.7) and 27.2 (SD: 12.9) and medians were 51% (range, 8.2\u201355.2) and 26 (range, 5.5\u2013148), respectively. Using a cut-off of 56% and 9, respectively, Kaplan-Meier survival curves showed an association of overall survival with Ki-67LI and MC. In addition to clinical stage, Ki-67LI maintained its prognostic value on multivariate analysis, supporting the role of Ki-67LI as an independent prognostic parameter. Based on these results, we propose a diagnostically applicable cut-off value of 56% for Ki-67LI as a prognostic parameter for canine splenic hemangiosarcoma.\n                  <\/jats:p>","DOI":"10.1177\/03009858231225507","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T07:45:35Z","timestamp":1705045535000},"page":"534-540","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Prognostic impact of Ki-67 in canine splenic hemangiosarcoma: A preliminary study"],"prefix":"10.1177","volume":"61","author":[{"given":"Elena","family":"Brigand\u00ec","sequence":"first","affiliation":[{"name":"University of Bologna, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Valenti","sequence":"additional","affiliation":[{"name":"Clinica Veterinaria Malpensa, Samarate, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2206-0384","authenticated-orcid":false,"given":"Barbara","family":"Bacci","sequence":"additional","affiliation":[{"name":"University of Bologna, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2694-905X","authenticated-orcid":false,"given":"Barbara","family":"Brunetti","sequence":"additional","affiliation":[{"name":"University of Bologna, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5203-7679","authenticated-orcid":false,"given":"Giancarlo","family":"Avallone","sequence":"additional","affiliation":[{"name":"University of Bologna, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,1,12]]},"reference":[{"key":"bibr1-03009858231225507","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.176"},{"key":"bibr2-03009858231225507","doi-asserted-by":"publisher","DOI":"10.1177\/0300985821999831"},{"issue":"9","key":"bibr3-03009858231225507","first-page":"967","volume":"59","author":"Batschinski K","year":"2018","journal-title":"Can Vet 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