{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:42:45Z","timestamp":1792791765267,"version":"4.5.0"},"reference-count":41,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T00:00:00Z","timestamp":1739318400000},"content-version":"vor","delay-in-days":366,"URL":"http:\/\/www.sagepub.com\/licence-information-for-chorus"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23H02380"],"award-info":[{"award-number":["23H02380"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009035","name":"Program on Open Innovation Platform with Enterprises, Research Institute and Academia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009035","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:p>Apolipoprotein C-III (ApoC-III) amyloidosis in humans is a hereditary amyloidosis caused by a D25V mutation in the APOC3 gene. This condition has only been reported in a French family and not in animals. We analyzed a 19-year-old white lion ( Panthera leo) that died in a Japanese safari park and found renal amyloidosis characterized by severe deposition confined to the renal corticomedullary border zone. Mass spectrometry-based proteomic analysis identified ApoC-III as a major component of renal amyloid deposits. Amyloid deposits were also positive for ApoC-III by immunohistochemistry. Based on these results, this case was diagnosed as ApoC-III amyloidosis for the first time in nonhuman animals. Five additional white lions were also tested for amyloid deposition retrospectively. ApoC-III amyloid deposition was detected in 3 white lions aged 19 to 21 years but not in 2 cases aged 0.5 and 10 years. Genetic analysis of white and regular-colored lions revealed that the APOC3 sequences of the lions were identical, regardless of amyloid deposition. These results suggest that ApoC-III amyloidosis in lions, unlike in humans, may not be a hereditary condition but an age-related condition. Interestingly, lion ApoC-III has a Val30 substitution compared with other species of Panthera that have Met30. Structural predictions suggest that the conformation of ApoC-III with Met30 and ApoC-III with Val30 are almost identical, but this substitution may alter the ability to bind to lipids. As with the D25V mutation in human ApoC-III, the Val30 substitution in lions may increase the proportion of free ApoC-III, leading to amyloid formation.<\/jats:p>","DOI":"10.1177\/03009858241230100","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T07:09:25Z","timestamp":1707721765000},"page":"574-581","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Apolipoprotein C-III amyloidosis in white lions (\n                    <i>Panthera leo<\/i>\n                    )"],"prefix":"10.1177","volume":"61","author":[{"given":"Natsumi","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Tokyo University of Agriculture and Technology, Fuchu-shi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Iwaide","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology, Fuchu-shi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroto","family":"Fukui","sequence":"additional","affiliation":[{"name":"Okayama University of Science, Imabari-shi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumi","family":"Une","sequence":"additional","affiliation":[{"name":"Okayama University of Science, Imabari-shi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Itoh","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology, Fuchu-shi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Hisada","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology, Fuchu-shi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-7842","authenticated-orcid":false,"given":"Tomoaki","family":"Murakami","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology, Fuchu-shi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,2,12]]},"reference":[{"key":"bibr1-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1177\/030098588402100106"},{"key":"bibr2-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1080\/13506129.2022.2147636"},{"key":"bibr3-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2016.04.003"},{"key":"bibr4-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0281822"},{"key":"bibr5-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800756200"},{"key":"bibr6-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn392"},{"key":"bibr7-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1998.tb03753.x"},{"key":"bibr8-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1638\/2019-0005"},{"key":"bibr9-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1002\/path.6150"},{"key":"bibr10-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2021.07.014"},{"key":"bibr11-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4390"},{"key":"bibr12-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1002\/jms.4348"},{"key":"bibr13-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz268"},{"key":"bibr14-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac240"},{"key":"bibr15-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1007\/s10072-020-04889-2"},{"key":"bibr16-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01488-1"},{"key":"bibr17-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1080\/13506129.2022.2120800"},{"key":"bibr18-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1177\/03009858211048650"},{"key":"bibr19-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1177\/03009858221148511"},{"key":"bibr20-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90057-4"},{"key":"bibr21-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810382089"},{"key":"bibr22-03009858241230100","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010319"},{"key":"bibr23-03009858241230100","doi-asserted-by":"publisher","DOI":"10.1177\/030098589703400602"},{"key":"bibr24-03009858241230100","doi-asserted-by":"crossref","unstructured":"Pollin TI, Damcott CM, Shen H, et al. A null mutation in human APOC3 confers a favorable plasma lipid profile and apparent cardioprotection. 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