{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:40:43Z","timestamp":1783111243358,"version":"3.54.6"},"reference-count":47,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Funding was provided by the sponsorship of the Southeastern Cooperative Wildlife Disease Study by the fish and wildlife agencies"},{"name":"Robert S. Toth Living Trust"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:p>Lymphoproliferative disease virus (LPDV) was first documented in wild turkeys in North America in 2009. LPDV infection is often subclinical but can manifest as lymphoid proliferation or round cell neoplasia. Despite high prevalence across many sampled areas corresponding to declining populations of wild turkeys, knowledge regarding LPDV pathogenesis, risk factors for disease development, and associated impacts on population dynamics are unknown. To understand transmission, viral shedding, and tissue tropism, we inoculated 21 domestic turkeys via the oral cavity, crop, nasal cavity, subcutis, or coelomic cavity. For 12 weeks, oropharyngeal swabs, cloacal swabs, and whole blood were collected weekly. At 1 week postinoculation, 3 turkeys (3\/21; 14%) had detectable LPDV proviral DNA in blood by polymerase chain reaction, and 10 developed DNAemia (50%; 10\/20) by 12 weeks. LPDV proviral DNA was intermittently detected in oropharyngeal and cloacal swabs. Splenomegaly was the most consistent gross finding in DNAemic birds (8\/11; 73%). Lymphoid hyperplasia in the spleen was the most significant microscopic finding (9\/11; 82%). Three turkeys (3\/11; 27%) developed round cell neoplasia characterized by sheets of pleomorphic, round to polygonal cells in the adrenal gland, bone marrow, skin, small intestine, and\/or spleen. LPDV was detected in the spleen and bone marrow from all turkeys with DNAemia and all neoplasms. Our study establishes that infection and disease with North American LPDV from wild turkeys can be experimentally reproduced in domestic turkeys, laying the groundwork for future investigations into LPDV pathogenesis, development of diagnostic techniques, and understanding the impacts of LPDV on wild turkey populations.<\/jats:p>","DOI":"10.1177\/03009858241231558","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T05:41:19Z","timestamp":1709098879000},"page":"562-573","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Experimental infection of domestic turkeys with lymphoproliferative disease virus of North American origin"],"prefix":"10.1177","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2181-018X","authenticated-orcid":false,"given":"Chloe C.","family":"Goodwin","sequence":"first","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"Department of Pathology, College of Veterinary Medicine, University of\rGeorgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayla G.","family":"Adcock","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew B.","family":"Allison","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic & Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark G.","family":"Ruder","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca L.","family":"Poulson","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole M.","family":"Nemeth","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"Department of Pathology, College of Veterinary Medicine, University of\rGeorgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"bibr1-03009858241231558","volume-title":"Avian histopathology","author":"Abdul-Aziz T.","year":"2016","edition":"4"},{"key":"bibr2-03009858241231558","first-page":"139","volume":"60","author":"Adcock KG","year":"2023","journal-title":"J Wildlife Dis"},{"key":"bibr3-03009858241231558","doi-asserted-by":"publisher","DOI":"10.1638\/2015-0037.1"},{"key":"bibr4-03009858241231558","doi-asserted-by":"publisher","DOI":"10.7589\/2016-06-137"},{"key":"bibr5-03009858241231558","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2013.11.037"},{"key":"bibr6-03009858241231558","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.539925"},{"key":"bibr7-03009858241231558","doi-asserted-by":"publisher","DOI":"10.1080\/03079457808418265"},{"key":"bibr8-03009858241231558","first-page":"506","author":"Broos H-W.","year":"1997","journal-title":"Tier\u00e4rztliche Umschau"},{"key":"bibr9-03009858241231558","first-page":"329","volume":"11","author":"Byrne ME","year":"2015","journal-title":"Proc Natl Wild Turkey Symp"},{"key":"bibr10-03009858241231558","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2022.102311"},{"key":"bibr11-03009858241231558","doi-asserted-by":"publisher","DOI":"10.1002\/jwmg.21883"},{"key":"bibr12-03009858241231558","doi-asserted-by":"publisher","DOI":"10.1080\/03079459508419050"},{"key":"bibr13-03009858241231558","doi-asserted-by":"publisher","DOI":"10.7589\/2015-05-129"},{"key":"bibr14-03009858241231558","first-page":"7","volume":"11","author":"Eriksen RE.","year":"2016","journal-title":"Proc Natl Wild Turkey Symp"},{"key":"bibr15-03009858241231558","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910310316"},{"key":"bibr16-03009858241231558","doi-asserted-by":"crossref","unstructured":"Gazit A, Yaniv A, Sarid R. 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