{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:32:10Z","timestamp":1777919530716,"version":"3.51.4"},"reference-count":107,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>High survivin expression has been correlated with poor outcomes in several canine tumors but not in soft tissue tumors (STTs). Survivin is a target gene of the Wnt\/\u03b2-catenin pathway, which is involved in human STT oncogenesis. Immunohistochemistry for survivin, \u03b2-catenin, and Ki-67 was performed on 41 canine perivascular wall tumors (cPWTs), and statistical associations of protein expression and histopathologic and clinical variables with clinical outcomes were investigated. Immunohistochemically, there was nuclear positivity (0.9%\u201312.2% of tumor cells) for survivin in 41\/41 (100%), cytoplasmic positivity (0 to &gt; 75% of tumor cells) for survivin in 31\/41 (76%), nuclear positivity (2.9%\u201367.2% of tumor cells) for \u03b2-catenin in 24\/41 (59%), and cytoplasmic positivity (0% to &gt; 75% of tumor cells) for \u03b2-catenin in 23\/41 (56%) of cPWTs. All tumors expressed nuclear Ki-67 (2.2%\u201323.5%). In univariate analysis and multivariate analysis (UA and MA, respectively), every 1% increase of nuclear survivin was associated with an increase of the instantaneous death risk by a factor of 1.15 [hazard ratio (HR) = 1.15; P = .007]. Higher nuclear survivin was associated with grade II\/III neoplasms ( P = .043). Expression of cytoplasmic survivin, nuclear and cytoplasmic \u03b2-catenin, and nuclear Ki-67 were not significantly associated with prognosis in UA nor MA. Tumor size was a significant prognostic factor for local recurrence in UA [subdistribution HR (SDHR) = 1.19; P = .02] and for reduced overall survival time in MA. According to UA and MA, a unitary increase of mitotic count was associated with an increase of the instantaneous death risk by a factor of 1.05 (HR = 1.05; P = .014). Nuclear survivin, mitotic count, and tumor size seem to be potential prognostic factors for cPWTs. In addition, survivin and \u03b2-catenin may represent promising therapeutic targets for cPWTs.<\/jats:p>","DOI":"10.1177\/03009858241246981","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T08:33:12Z","timestamp":1715329992000},"page":"912-927","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Survivin, \u03b2-catenin, and ki-67 immunohistochemical expression in canine perivascular wall tumors: Preliminary assessment of prognostic significance"],"prefix":"10.1177","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7617-4371","authenticated-orcid":false,"given":"Francesco","family":"Godizzi","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2578-4409","authenticated-orcid":false,"given":"Federico","family":"Armando","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8506-2005","authenticated-orcid":false,"given":"Patrizia","family":"Boracchi","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences \u201cLuigi Sacco\u201d, University of Milan, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5203-7679","authenticated-orcid":false,"given":"Giancarlo","family":"Avallone","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, Ozzano dell\u2019Emilia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2726-0366","authenticated-orcid":false,"given":"Damiano","family":"Stefanello","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lavinia E.","family":"Chiti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3729-528X","authenticated-orcid":false,"given":"Andrea","family":"Cappelleri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, Italy"},{"name":"Mouse and Animal Pathology Laboratory (MAPLab), Fondazione UniMi, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarissa","family":"Zamboni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Dell\u2019Aere","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attilio","family":"Corradi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Parma, Parma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3672-4612","authenticated-orcid":false,"given":"Paola","family":"Roccabianca","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,5,10]]},"reference":[{"key":"bibr1-03009858241246981","doi-asserted-by":"publisher","DOI":"10.3390\/ani10122318"},{"key":"bibr2-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclinepi.2016.11.017"},{"key":"bibr3-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1002\/sim.7501"},{"key":"bibr4-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985813503565"},{"key":"bibr5-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1354\/vp.44-5-607"},{"key":"bibr6-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985815575050"},{"key":"bibr7-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12559"},{"key":"bibr8-03009858241246981","doi-asserted-by":"publisher","DOI":"10.2460\/javma.230.4.548"},{"key":"bibr9-03009858241246981","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2004.224.887"},{"key":"bibr10-03009858241246981","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092125"},{"key":"bibr11-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-013-2856-0"},{"key":"bibr12-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985818794161"},{"key":"bibr13-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00822.x"},{"key":"bibr14-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12211"},{"key":"bibr15-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-8-78"},{"key":"bibr16-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985814529312"},{"key":"bibr17-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/03009858211014174"},{"key":"bibr18-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12556"},{"key":"bibr19-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/2036361318813431"},{"key":"bibr20-03009858241246981","doi-asserted-by":"publisher","DOI":"10.7150\/jca.13332"},{"key":"bibr21-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-020-0627-5"},{"key":"bibr22-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12673"},{"key":"bibr23-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2012.07.001"},{"key":"bibr24-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1002\/jso.22134"},{"key":"bibr25-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.10.018"},{"key":"bibr26-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/761034"},{"key":"bibr27-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810388820"},{"key":"bibr28-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2015.12.005"},{"key":"bibr29-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.183277"},{"key":"bibr30-03009858241246981","doi-asserted-by":"publisher","DOI":"10.2460\/javma.228.7.1053"},{"key":"bibr31-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-13-209"},{"key":"bibr32-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800203"},{"key":"bibr33-03009858241246981","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21144852"},{"key":"bibr34-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1038\/s41409-019-0679-x"},{"key":"bibr35-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985818789466"},{"key":"bibr36-03009858241246981","unstructured":"Gray B. Cmprsk: subdistribution analysis of competing risks (R Package Version 2.2-11), 2022. https:\/\/CRAN.R-project.org\/package=cmprsk."},{"key":"bibr37-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.500"},{"key":"bibr38-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985809359303"},{"key":"bibr39-03009858241246981","doi-asserted-by":"publisher","DOI":"10.4135\/9781412985628"},{"key":"bibr40-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1053\/he.2000.6496"},{"key":"bibr41-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-1956-z"},{"key":"bibr42-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2006.00473.x"},{"key":"bibr43-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20011120)95:6<360::AID-IJC1063>3.0.CO;2-1"},{"key":"bibr44-03009858241246981","first-page":"1098","volume":"9","author":"Kappler M","year":"2003","journal-title":"Clin Cancer Res"},{"key":"bibr45-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cgt.7701090"},{"key":"bibr46-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13910-4"},{"key":"bibr47-03009858241246981","unstructured":"Koenker R. Quantreg: quantile regression (R Package Version 5.94), 2022. https:\/\/CRAN.R-project.org\/package=quantreg."},{"key":"bibr48-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880181"},{"key":"bibr49-03009858241246981","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1997.211.09.1147"},{"key":"bibr50-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2014.161"},{"key":"bibr51-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1473-8"},{"key":"bibr52-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20768"},{"key":"bibr53-03009858241246981","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41392-021-00710-4","volume":"7","author":"Liu J","year":"2022","journal-title":"Signal Transduct Target Ther"},{"key":"bibr54-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-16-0585"},{"key":"bibr55-03009858241246981","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13215521"},{"key":"bibr56-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1002\/med.21547"},{"key":"bibr57-03009858241246981","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2000.217.205"},{"key":"bibr58-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1354\/vp.08-VP-0277-M-FL"},{"key":"bibr59-03009858241246981","unstructured":"Meuten DJ, Bertram CA, Moore FM, et al. Mitotic count guideline (Version 1.0). Veterinary Cancer Guidelines and Protocols. http:\/\/vetcancerprotocols.org."},{"key":"bibr60-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985815593349"},{"key":"bibr61-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-003-0255-2"},{"key":"bibr62-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2018.09.001"},{"key":"bibr63-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2020.10.025"},{"key":"bibr64-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2008.02.001"},{"key":"bibr65-03009858241246981","first-page":"751","volume":"21","author":"Nowak M","year":"2007","journal-title":"In Vivo"},{"key":"bibr66-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.19-0029"},{"key":"bibr67-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/0300985812456213"},{"key":"bibr68-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/0895-4356(95)00048-8"},{"key":"bibr69-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-83"},{"key":"bibr70-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29027"},{"key":"bibr71-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2007.00150.x"},{"key":"bibr72-03009858241246981","unstructured":"R Core Team. R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing; 2022. https:\/\/www.R-project.org."},{"key":"bibr73-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2008.0143.x"},{"key":"bibr74-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/03009858221079666"},{"key":"bibr75-03009858241246981","first-page":"232","volume-title":"Surgical Pathology of Tumors of Domestic Animals. Volume 3: Tumors of Soft Tissue","volume":"2020","author":"Roccabianca P"},{"key":"bibr76-03009858241246981","first-page":"206","volume-title":"Surgical Pathology of Tumors of Domestic Animals. Volume 3: Tumors of Soft Tissue","volume":"2020","author":"Roccabianca P"},{"key":"bibr77-03009858241246981","first-page":"214","volume-title":"Surgical Pathology of Tumors of Domestic Animals. Volume 3: Tumors of Soft Tissue","volume":"2020","author":"Roccabianca P"},{"key":"bibr78-03009858241246981","volume-title":"Surgical Pathology of Tumors of Domestic Animals. Volume 3: Tumors of Soft Tissue","author":"Roccabianca P","year":"2020"},{"key":"bibr79-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3865-5"},{"key":"bibr80-03009858241246981","doi-asserted-by":"publisher","DOI":"10.2174\/157016307782109652"},{"key":"bibr81-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2006.tb02835.x"},{"key":"bibr82-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/0197-2456(96)00075-X"},{"key":"bibr83-03009858241246981","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2005.227.1442"},{"key":"bibr84-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20951"},{"key":"bibr85-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12104"},{"key":"bibr86-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-2315"},{"key":"bibr87-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-016-9809-7"},{"key":"bibr88-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/1040638717743280"},{"key":"bibr89-03009858241246981","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci10010001"},{"key":"bibr90-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1515\/pjvs-2017-0040"},{"key":"bibr91-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1515\/pjvs-2015-0015"},{"key":"bibr92-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2011.00822.x"},{"key":"bibr93-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203665"},{"key":"bibr94-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-65"},{"key":"bibr95-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-020-02317-3"},{"key":"bibr96-03009858241246981","unstructured":"Therneau T. A package for survival analysis in R (R Package Version 3.4-0), 2022. https:\/\/CRAN.R-project.org\/package=survival."},{"key":"bibr97-03009858241246981","doi-asserted-by":"publisher","DOI":"10.4172\/2168-9431.1000139"},{"key":"bibr98-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-021-03871-5"},{"key":"bibr99-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1038\/70487"},{"key":"bibr100-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.03.010"},{"key":"bibr101-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1177\/1471082X18759142"},{"key":"bibr102-03009858241246981","first-page":"132","author":"Wheatley SP","year":"2019","journal-title":"J Cell Sci"},{"key":"bibr103-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2009.12.004"},{"key":"bibr104-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079810"},{"key":"bibr105-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.05.003"},{"key":"bibr106-03009858241246981","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2015.02.003"},{"key":"bibr107-03009858241246981","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.13.6264"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858241246981","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03009858241246981","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858241246981","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T19:05:19Z","timestamp":1777662319000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03009858241246981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,10]]},"references-count":107,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1177\/03009858241246981"],"URL":"https:\/\/doi.org\/10.1177\/03009858241246981","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,10]]}}}