{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:32:09Z","timestamp":1777919529912,"version":"3.51.4"},"reference-count":48,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T00:00:00Z","timestamp":1715904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["PRIN 2017 Prot. 20175XHBPN"],"award-info":[{"award-number":["PRIN 2017 Prot. 20175XHBPN"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>\n                    The Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mouse is a well-established model of X-linked hyper-immunoglobulin M (IgM) syndrome, an immunodeficiency disorder of human beings characterized by the lack of expression of the CD40 ligand (CD40L) on activated T-cells, predisposing to infections with opportunistic pathogens like Pneumocystis jirovecii. The aim of our study was to describe the pulmonary lesions in Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice experimentally infected with Pneumocystis murina, in comparison with naturally infected severe combined immunodeficient (SCID) mice. Formalin-fixed paraffin-embedded lungs from 26 Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    , 11 SCID, and 5 uninfected Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice were examined by histology and immunohistochemistry for the presence of the pathogen and for leukocyte populations (CD3, CD4, CD45R\/B220, CD8a, Iba-1, Ly-6G, CD206, MHC II, and NKp46\/NCR1). Infection was confirmed by immunohistochemistry in 18\/26 (69%) Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice and in 11\/11 (100%) SCID mice. Fourteen out of 26 (54%) Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice had interstitial pneumonia. Twenty-three out of 26 (88%) Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice had peribronchiolar\/perivascular lymphoplasmacytic infiltrates, rich in B-cells and Mott cells. Acidophilic macrophage pneumonia was additionally found in 20\/26 (77%) Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice. Only 4\/11 (36%) SCID mice had interstitial pneumonia, but no peribronchiolar\/perivascular infiltrates or acidophilic macrophage pneumonia were observed in this strain. This study represents the first description of pulmonary histopathological lesions in Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice infected with P. murina. We speculate that the singular characteristics of the inflammatory infiltrates observed in Cd40l\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice could be explained by the specific immune phenotype of the model.\n                  <\/jats:p>","DOI":"10.1177\/03009858241252409","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T06:45:13Z","timestamp":1715928313000},"page":"988-997","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>Pneumocystis murina<\/i>\n                    lesions in lungs of experimentally infected Cd40l\n                    <sup>\u2013\/\u2013<\/sup>\n                    mice"],"prefix":"10.1177","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3729-528X","authenticated-orcid":false,"given":"Andrea","family":"Cappelleri","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine 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Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1851-1429","authenticated-orcid":false,"given":"Valentina","family":"Capo","sequence":"additional","affiliation":[{"name":"San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy"},{"name":"National Research Council, Institute of Genetic and Biomedical Research, Milan Unit, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Zecchillo","sequence":"additional","affiliation":[{"name":"San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy"},{"name":"National Research Council, Institute of Genetic and Biomedical Research, Milan Unit, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa","family":"Albano","sequence":"additional","affiliation":[{"name":"San Raffaele Telethon Institute for Gene Therapy 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