{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:53:05Z","timestamp":1787320385447,"version":"build-2736575974"},"reference-count":16,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2024,12,7]],"date-time":"2024-12-07T00:00:00Z","timestamp":1733529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:p>\n                    The American alligator (\n                    <jats:italic toggle=\"yes\">Alligator mississippiensis<\/jats:italic>\n                    ) is a keystone species of the southeastern United States. In December of 2022, a free-ranging American alligator was found stuporous and tetraparetic. On postmortem evaluation, lesions were limited to the central nervous system, consisting of prominent perivascular cuffs of lymphocytes and histiocytes that extended into the surrounding neuroparenchyma along with gliosis. Next-generation sequencing of the affected brain identified the presence of a piscichuvirus closely related to the freshwater turtle neurovirus 1 (FTuNV-1) recently reported in an alligator snapping turtle with similar microscopic lesions.\n                    <jats:italic toggle=\"yes\">In situ<\/jats:italic>\n                    hybridization using zz-probes that recognize FTuNV-1 identified widespread hybridization signal in neurons and glial cells in the alligator\u2019s brain and spinal cord. This case represents only the second association of piscichuviruses with vertebrate disease. Moreover, it highlights the potential for disease transmission between different orders (Crocodylia and Testudines) of free-ranging aquatic reptiles that share similar habitats in the United States.\n                  <\/jats:p>","DOI":"10.1177\/03009858241300559","type":"journal-article","created":{"date-parts":[[2024,12,7]],"date-time":"2024-12-07T06:08:36Z","timestamp":1733551716000},"page":"586-591","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Piscichuvirus-associated meningoencephalomyelitis in a free-ranging American alligator (\n                    <i>Alligator mississippiensis<\/i>\n                    )"],"prefix":"10.1177","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7029-0600","authenticated-orcid":false,"given":"Peres R.","family":"Badial","sequence":"first","affiliation":[{"name":"University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6745-0140","authenticated-orcid":false,"given":"Weerapong","family":"Laovechprasit","sequence":"additional","affiliation":[{"name":"University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carley","family":"Laue","sequence":"additional","affiliation":[{"name":"University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian A.","family":"Stacy","sequence":"additional","affiliation":[{"name":"University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7661-2631","authenticated-orcid":false,"given":"James B.","family":"Stanton","sequence":"additional","affiliation":[{"name":"University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuttichantran","family":"Subramaniam","sequence":"additional","affiliation":[{"name":"University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6951-6070","authenticated-orcid":false,"given":"Robert J.","family":"Ossiboff","sequence":"additional","affiliation":[{"name":"University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,12,7]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092476"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8667(2002)014<0149:PRASAM>2.0.CO;2"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01954-21"},{"key":"e_1_3_3_6_2","first-page":"9","volume-title":"Ranaviruses: Lethal Pathogens of Ectothermic Vertebrates","author":"Duffus ALJ","unstructured":"Duffus ALJ, Waltzek TB, St\u00f6hr AC, et al. Distribution and host range of Ranaviruses. In: Gray MJ, Chinchar VG, eds. Ranaviruses: Lethal Pathogens of Ectothermic Vertebrates. London, UK: Springer International Publishing; 2015:9\u201357."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.09.013"},{"key":"e_1_3_3_8_2","unstructured":"ICTV. International Committee on Taxonomy of Viruses. Accessed July 1 2024. https:\/\/ictv.global\/taxonomy."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-41.1.96"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkf436"},{"issue":"2","key":"e_1_3_3_11_2","first-page":"280","article-title":"Piscichuvirus-associated severe meningoencephalomyelitis in aquatic turtles, United States, 2009\u20132021","volume":"30","author":"Laovechprasit W","year":"2024","unstructured":"Laovechprasit W, Young KT, Stacy BA, et al. Piscichuvirus-associated severe meningoencephalomyelitis in aquatic turtles, United States, 2009\u20132021. Emerg Infect Dis. 2024;30(2):280\u2013288.","journal-title":"Emerg Infect Dis"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05378"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0907.030085"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_3_3_15_2","first-page":"1","volume-title":"Diet and Condition of American Alligators (Alligator mississippiensis) in Three Central Florida Lakes","author":"Rice AN.","unstructured":"Rice AN. Diet and Condition of American Alligators (Alligator mississippiensis) in Three Central Florida Lakes [master\u2019s thesis]. Gainesville, FL: University of Florida. 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