{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:32:44Z","timestamp":1777919564171,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:p>Liver-type fatty acid-binding protein (L-FABP) is expressed by several tissues, plays a role in fatty acid metabolism, and has antioxidant effects. Its renal expression is upregulated by stress. Urinary L-FABP (uL-FABP) is a promising kidney biomarker in people for detection of early acute and chronic kidney disease (CKD) and as a marker for progression in patients with glomerulonephritis. However, data on canine uL-FABP are currently limited. This prospective study was designed to examine canine tissue expression of L-FABP and to validate an ELISA to quantify uL-FABP in older dogs with or without early signs of CKD. Tissues of 4 recently euthanized dogs and 117 urine samples of 73 client-owned older dogs undergoing health screening were evaluated in the study. Immunohistochemistry was performed on kidney and liver tissues. Analytical validation of a commercially available ELISA for measurement of L-FABP in canine urine was performed (limit of detection, imprecision, specificity). The ELISA was used to measure L-FABP in stored urine samples from a cohort of older dogs. Dogs were found to express L-FABP, mostly in proximal tubular epithelial cells and in the periportal hepatocytes of the liver. Assay validation revealed poor sensitivity and imprecision for measurement of canine uL-FABP. Of the 117 urine samples analyzed, 98 were below the limit of detection (LOD; 7.30 ng\/mL) and a further 5 were below the limit of quantification (LOQ; 16.20 ng\/mL). The proximal tubules of dog kidneys express L-FABP, but the value of uL-FABP as tubular marker in older dogs warrants further study.<\/jats:p>","DOI":"10.1177\/03009858251315106","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T04:36:51Z","timestamp":1739248611000},"page":"719-727","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Tissue expression and urinary excretion of liver-type fatty acid binding protein in older dogs with or without early signs of chronic kidney disease"],"prefix":"10.1177","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1884-9366","authenticated-orcid":false,"given":"Sofie","family":"Marynissen","sequence":"first","affiliation":[{"name":"Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristel","family":"Demeyere","sequence":"additional","affiliation":[{"name":"Lab of Biochemistry, Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Daminet","sequence":"additional","affiliation":[{"name":"Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyne","family":"Meyer","sequence":"additional","affiliation":[{"name":"Lab of Biochemistry, Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koen","family":"Chiers","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Paepe","sequence":"additional","affiliation":[{"name":"Small Animal Department, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"49","article-title":"Limit of blank, limit of detection and limit of quantitation","volume":"29","author":"Armbruster DA","year":"2008","unstructured":"Armbruster DA, Pry T. 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