{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:10:39Z","timestamp":1791231039978,"version":"4.1.0"},"reference-count":40,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2020-06472"],"award-info":[{"award-number":["RGPIN-2020-06472"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["Vanier Canadian Graduate Scholarship"],"award-info":[{"award-number":["Vanier Canadian Graduate Scholarship"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014116","name":"Ontario Veterinary College, University of Guelph","doi-asserted-by":"publisher","award":["OVC Fellowship"],"award-info":[{"award-number":["OVC Fellowship"]}],"id":[{"id":"10.13039\/100014116","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015900","name":"OVC Pet Trust","doi-asserted-by":"publisher","award":["055849"],"award-info":[{"award-number":["055849"]}],"id":[{"id":"10.13039\/100015900","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:p>\n                    Splenic masses are common in dogs and vary dramatically in their clinical behavior. Clinically, and even with histology, it can be challenging to differentiate between benign and malignant splenic masses. Hemangiosarcoma (HSA), the most common malignancy of the spleen, is a very aggressive tumor with a poor prognosis. We hypothesize that microRNAs (miRNAs) in mass tissue and serum can differentiate between HSA and other splenic masses. Fifty-nine miRNAs were investigated by reverse-transcription followed by real-time quantitative polymerase chain reaction (RT-qPCR) in serum and\/or tissue from dogs with HSAs (serum\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 24 and tissue\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 17; postsplenectomy serum\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 11), lymphomas (serum\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 8 and tissue\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 11), nonangiomatous nonlymphomatous sarcomas (serum\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 6 and tissue\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 10), histiocytic sarcomas (tissue\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 4), benign splenic masses (myelolipomas, nodular hyperplasia, and hematomas; total serum\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 21 and total tissue\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 35), and normal dogs (serum\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 14 and tissue\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 7). Numerous miRNAs were differentially expressed in serum and tissue of HSA cases compared to those with other splenic masses or normal spleens. In serum, our 5-miRNA model (miR-135a-5p, miR-10a, miR-450b, miR-152-3p, and miR-126-5p) accurately classified 100% (24\/24) of dogs with HSA from normal dogs and those with a benign splenic mass (recall = 1 for HSA). The overall accuracy of the model was 86%. In HSA and benign splenic mass tissues, our 3-miRNA model (miR-126-5p, miR-502-3p, and miR-452-5p) accurately classified 96% of the cases. This study demonstrates the utility of miRNA models in serum and tissue for screening and diagnosis of HSA in dogs. Future studies include the evaluation of prospective and prediagnosis serum samples.\n                  <\/jats:p>","DOI":"10.1177\/03009858251317466","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T06:36:31Z","timestamp":1739946991000},"page":"659-671","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["MicroRNAs in serum and tissue can differentiate splenic hemangiosarcoma from other splenic masses in dogs"],"prefix":"10.1177","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8328-1031","authenticated-orcid":false,"given":"Latasha","family":"Ludwig","sequence":"first","affiliation":[{"name":"University of Guelph, Guelph, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Treleaven","sequence":"additional","affiliation":[{"name":"University of Guelph, Guelph, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlene","family":"Khachadoorian","sequence":"additional","affiliation":[{"name":"University of Guelph, Guelph, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0559-3728","authenticated-orcid":false,"given":"Brigitte","family":"Degasperi","sequence":"additional","affiliation":[{"name":"University of Veterinary Medicine, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Walter","sequence":"additional","affiliation":[{"name":"University of Veterinary Medicine, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deirdre","family":"Stuart","sequence":"additional","affiliation":[{"name":"University of Guelph, Guelph, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Foster","sequence":"additional","affiliation":[{"name":"University of Guelph, Guelph, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert Darren","family":"Wood","sequence":"additional","affiliation":[{"name":"University of Guelph, Guelph, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Ayesha","family":"Ali","sequence":"additional","affiliation":[{"name":"University of Guelph, Guelph, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey A.","family":"Wood","sequence":"additional","affiliation":[{"name":"University of Guelph, Guelph, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03445-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.4155\/bio.13.344"},{"issue":"9","key":"e_1_3_3_5_2","first-page":"967","article-title":"Canine visceral hemangiosarcoma treated with surgery alone or surgery and doxorubicin: 37 cases (2005-2014)","volume":"59","author":"Batschinski K","year":"2018","unstructured":"Batschinski K, Nobre A, Vargas-Mendez E, et al. 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