{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:32:51Z","timestamp":1777919571168,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T00:00:00Z","timestamp":1745193600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Recovery and Resilience Facility","award":["RRF-2.3.1-21"],"award-info":[{"award-number":["RRF-2.3.1-21"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:p>\n                    The diagnosis of porcine circovirus 2 (PCV-2) systemic disease is based on clinical signs, microscopic lesions, and semiquantitative PCV-2 assessment by in situ visualization of immunohistochemistry (IHC) or in situ hybridization labeling within lymphoid tissue lesions. We aimed to digitally quantify IHC-labeled PCV-2-infected cells in porcine lymph nodes with natural PCV-2 systemic disease (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    = 53) and to compare these quantitative results with the subjective manual method, and also with the viral DNA copy numbers of the corresponding formalin-fixed, paraffin-embedded tissue blocks. In total, 47 of 54 (87%) cases had the same manual score by 2 independent observers. Infected cell counts increased with manual score severity, and their count could be matched to the manual method. Infected cell odds showed a significant strong positive correlation with the viral copy numbers (\u03c1 = 0.8461,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; .001). Digital image analysis is efficient for PCV-2 IHC signal quantification and can make case assessment more objective and reproducible.\n                  <\/jats:p>","DOI":"10.1177\/03009858251331121","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T08:50:26Z","timestamp":1745225426000},"page":"714-718","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Digital quantification of porcine circovirus 2 (PCV-2)-infected cells in lymph nodes of pigs with natural PCV-2 systemic disease shows strong association with manual scoring and quantitative PCR"],"prefix":"10.1177","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4844-4543","authenticated-orcid":false,"given":"D\u00e1vid G.","family":"Horv\u00e1th","sequence":"first","affiliation":[{"name":"University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilla","family":"D\u00e9nes","sequence":"additional","affiliation":[{"name":"University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Igriczi","sequence":"additional","affiliation":[{"name":"University of Veterinary Medicine, Budapest, Hungary"},{"name":"National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rton","family":"Papp","sequence":"additional","affiliation":[{"name":"University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edctor","family":"Hidalgo-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Universitat Aut\u00f3noma de Barcelona, Bellaterra, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1539-7261","authenticated-orcid":false,"given":"Joaquim","family":"Segal\u00e9s","sequence":"additional","affiliation":[{"name":"Universitat Aut\u00f3noma de Barcelona, Bellaterra, Spain"},{"name":"Institute of Agrifood Research and Technology, Bellaterra, Barcelona, Spain"},{"name":"WOAH Collaborating Center for Research and Control of Emerging and Re-Emerging Pig Diseases, Bellaterra, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyula","family":"Balka","sequence":"additional","affiliation":[{"name":"University of Veterinary Medicine, Budapest, Hungary"},{"name":"National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,4,21]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-018-0123-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0386-RA"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/03009858221105053"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2022.110457"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.08.014"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci8100235"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/03009858221140818"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani13050830"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-1283"},{"key":"e_1_3_3_12_2","first-page":"965","article-title":"Digital microscopy\u2014the upcoming revolution in histopathology teaching, diagnostics, research and quality assurance","volume":"2","author":"Kren\u00e1cs T","year":"2010","unstructured":"Kren\u00e1cs T, Zsakovics I, Micsik T, et al. Digital microscopy\u2014the upcoming revolution in histopathology teaching, diagnostics, research and quality assurance. Microsc Sci Technol Appl Educ. 2010;2:965\u2013977.","journal-title":"Microsc Sci Technol Appl Educ"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.712944"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2003.12.007"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres\/2010003"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1053\/jcpa.1998.0258"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/03009858231180484"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.10.007"},{"key":"e_1_3_3_19_2","first-page":"473","volume-title":"Diseases of Swine","author":"Segal\u00e9s J","unstructured":"Segal\u00e9s J, Allan GM, Domingo M. Chapter 30 Circoviruses. In: Zimmerman JJ, ed. Diseases of Swine. 11th ed, Hoboken, NJ: Wiley Blackwell; 2018:473\u2013487.","edition":"11"},{"key":"e_1_3_3_20_2","volume-title":"21st International Pig Veterinary Society (IPVS) Congress;","author":"Segal\u00e9s J","year":"2010","unstructured":"Segal\u00e9s J, Bel\u00e1k K, Bille-Hansen V, et al. International immunohistochemistry ring trial for the detection of porcine circovirus type 2 (PCV2) antigen on formalin-fixed, paraffin-embedded tissues. 21st International Pig Veterinary Society (IPVS) Congress; July 2010; Vancouver, BC."},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci9030110"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1177\/03009858211040484"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858251331121","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03009858251331121","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858251331121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T19:06:19Z","timestamp":1777662379000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03009858251331121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,21]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1177\/03009858251331121"],"URL":"https:\/\/doi.org\/10.1177\/03009858251331121","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,21]]}}}