{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:06:31Z","timestamp":1787018791610,"version":"build-2736575974"},"reference-count":56,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"The Second Century Fund (C2F), Chulalongkorn University"},{"name":"Thailand Science Research and Innovation Fund Chulalongkorn University","award":["HEA_FF_68_051_3100_009"],"award-info":[{"award-number":["HEA_FF_68_051_3100_009"]}]},{"DOI":"10.13039\/501100004704","name":"national research council of thailand","doi-asserted-by":"publisher","award":["NRCT5-RGJ63001-013"],"award-info":[{"award-number":["NRCT5-RGJ63001-013"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Lymphocryptoviruses (LCVs), members of the\n                    <jats:italic toggle=\"yes\">Gammaherpesvirinae<\/jats:italic>\n                    subfamily, are associated with chronic fibrotic diseases in humans and animals. This study investigated LCV infection in 7 orangutans (\n                    <jats:italic toggle=\"yes\">Pongo pygmaeus<\/jats:italic>\n                    ), including 2 recent cases with severe respiratory disease and 5 archival cases analyzed retrospectively. Comprehensive necropsies were conducted on the recent cases, and all 7 underwent histopathological evaluation, quantitative polymerase chain reaction (qPCR), and in situ hybridization (ISH). LCV was detected in 3 cases (43%) by qPCR, including the 2 recent cases and 1 archival case. The highest viral loads, as determined by the qPCR, were observed in the lungs of positive cases. Histological examination revealed significant pulmonary interstitial fibrosis and vascular remodeling in all LCV-positive cases, and type II pneumocyte hypertrophy was most prominent in an acute case. Masson\u2019s trichrome staining confirmed varying degrees of fibrosis in the pulmonary interstitium and perivascular stroma. LCV DNA, identified through ISH, was observed in alveolar macrophages, endothelial cells of pulmonary capillaries, and spindle cells within fibrotic regions of the lung and heart. Viral isolation from lung tissue in 1 recent case was successful, demonstrating cytopathic effects in Vero cells. Genetic sequencing revealed a novel LCV strain closely related to\n                    <jats:italic toggle=\"yes\">Pongo pygmaeus lymphocryptovirus-1<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Macacine lymphocryptoviruses<\/jats:italic>\n                    . These findings link LCV infection to fibrotic pulmonary lesions in orangutans. Further studies are essential to elucidate the mechanisms of LCV-related disease and its implications for wildlife conservation.\n                  <\/jats:p>","DOI":"10.1177\/03009858251361135","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T07:34:39Z","timestamp":1756193679000},"page":"63-73","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Lymphocryptovirus infection in orangutans associated with interstitial and perivascular pulmonary fibrosis"],"prefix":"10.1177","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5148-4696","authenticated-orcid":false,"given":"Chutchai","family":"Piewbang","sequence":"first","affiliation":[{"name":"Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6717-5054","authenticated-orcid":false,"given":"Aisyah Nikmatuz","family":"Zahro","sequence":"additional","affiliation":[{"name":"Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-7071","authenticated-orcid":false,"given":"Panida","family":"Poonsin","sequence":"additional","affiliation":[{"name":"Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3199-6539","authenticated-orcid":false,"given":"Sitthichok","family":"Lacharoje","sequence":"additional","affiliation":[{"name":"Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6507-6020","authenticated-orcid":false,"given":"Sawang","family":"Kesdangsakonwut","sequence":"additional","affiliation":[{"name":"Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7465-0696","authenticated-orcid":false,"given":"Nathamon","family":"Kosoltanapiwat","sequence":"additional","affiliation":[{"name":"Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1895-8992","authenticated-orcid":false,"given":"Tanit","family":"Kasantikul","sequence":"additional","affiliation":[{"name":"Michigan State University, East Lansing, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4888-2677","authenticated-orcid":false,"given":"Somporn","family":"Techangamsuwan","sequence":"additional","affiliation":[{"name":"Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-092917-043227"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002962"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055715"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.11.9206-9212.1999"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00448-w"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.08.002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.08.026"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.02.014"},{"key":"e_1_3_2_10_2","first-page":"225","article-title":"Murine gammaherpesvirus 68: a small animal model for gammaherpesvirus-associated diseases","volume":"2017","author":"Dong S","unstructured":"Dong S, Forrest JC, Liang X. 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