{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T07:28:11Z","timestamp":1793518091598,"version":"4.5.0"},"reference-count":38,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100009964","name":"American Association of Zoo Veterinarians","doi-asserted-by":"publisher","award":["Wild Animal Health Fund"],"award-info":[{"award-number":["Wild Animal Health Fund"]}],"id":[{"id":"10.13039\/100009964","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000202","name":"U.S. Fish and Wildlife Service","doi-asserted-by":"publisher","award":["Wildlife Restoration Grant"],"award-info":[{"award-number":["Wildlife Restoration Grant"]}],"id":[{"id":"10.13039\/100000202","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:p>\n                    Canine parvovirus 2a (CPV-2a) causes severe enteritis and diarrhea in unvaccinated domestic puppies and wild mesocarnivores, such as raccoons (\n                    <jats:italic toggle=\"yes\">Procyon lotor<\/jats:italic>\n                    ). A retrospective and prospective study was conducted to characterize CPV-induced encephalomyelitis in mountain lions (\n                    <jats:italic toggle=\"yes\">Puma concolor<\/jats:italic>\n                    ) from California. Four free-ranging juvenile mountain lions were euthanized due to severe neurological signs. Microscopically, multifocal neuronal necrosis, lymphoplasmacytic perivascular cuffing, and gliosis were primarily observed in the gray matter of the brain and spinal cord in all the studied animals. Rare intranuclear inclusion bodies were observed in the brainstem of 1 animal. All animals lacked microscopic and macroscopic evidence of enteritis. The association between the lesions and CPV-2 was confirmed by quantitative polymerase chain reaction (qPCR) and immunohistochemistry, which revealed the presence of viral DNA in nervous tissue and antigen in numerous necrotic neurons, respectively. Detection of\n                    <jats:italic toggle=\"yes\">CPV-2<\/jats:italic>\n                    mRNA by quantitative reverse transcription-PCR and nucleic acids by in situ hybridization indicated viral transcription in the necrotic neurons. Negative-contrast transmission electron microscopy revealed Parvoviridae particles in the brain, suggesting viral replication and assembly. Molecular sequencing in 2 cases detected a CPV-2a variant with amino acid substitutions previously observed in other wild carnivores. Although rare, CPV-2a is a potential cause of neurological signs in juvenile mountain lions without evidence of diarrhea or enteritis.\n                  <\/jats:p>","DOI":"10.1177\/03009858261465442","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T04:26:34Z","timestamp":1785471994000},"page":"1063-1071","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Polioencephalomyelitis and neuronal necrosis caused by canine parvovirus-2a variant in free-ranging juvenile mountain lions (\n                    <i>Puma concolor<\/i>\n                    )"],"prefix":"10.1177","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3600-0988","authenticated-orcid":false,"given":"Omar A.","family":"Gonzales-Viera","sequence":"first","affiliation":[{"name":"University of California, Davis, Davis, CA"},{"name":"Universidad Nacional Mayor de San Marcos, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1870-9945","authenticated-orcid":false,"given":"An\u00edbal G.","family":"Armien","sequence":"additional","affiliation":[{"name":"University of California, Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0681-1878","authenticated-orcid":false,"given":"Francisco A.","family":"Uzal","sequence":"additional","affiliation":[{"name":"University of California, Davis, San Bernardino, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6178-4801","authenticated-orcid":false,"given":"Javier","family":"Asin","sequence":"additional","affiliation":[{"name":"University of California, Davis, San Bernardino, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliann","family":"Beingesser","sequence":"additional","affiliation":[{"name":"University of California, Davis, San Bernardino, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha M.","family":"Barnum","sequence":"additional","affiliation":[{"name":"University of California, Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwafemi F.","family":"Adu","sequence":"additional","affiliation":[{"name":"Cornell University, Ithaca, NY"},{"name":"University of Alberta, Edmonton, AB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin R.","family":"Parrish","sequence":"additional","affiliation":[{"name":"Cornell University, Ithaca, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7612-6713","authenticated-orcid":false,"given":"Asl\u0131","family":"Mete","sequence":"additional","affiliation":[{"name":"University of California, Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Rudd","sequence":"additional","affiliation":[{"name":"Center for Natural Lands Management, Temecula, CA"},{"name":"California Department of Fish and Wildlife, Rancho Cordova, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deana L.","family":"Clifford","sequence":"additional","affiliation":[{"name":"California Department of Fish and Wildlife, Rancho Cordova, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06187-11"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004475"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(01)00770-5"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879400600301"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/03009858231180488"},{"key":"e_1_3_3_7_2","unstructured":"California Fish and Game Commission. Mountain Lions (Puma concolor) in Southern and Central Coastal California are Threatened or Endangered. State of California Department of Fish and Wildlife; 2020. Accessed September 25 2025. https:\/\/wildlife.ca.gov\/Conservation\/Mammals\/Mountain-Lion"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00460-18"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.11.005"},{"key":"e_1_3_3_10_2","volume-title":"Veterinary Neuroanatomy and Clinical Neurology","author":"De Lahunta A","year":"2008","unstructured":"De Lahunta A, Glass E. Development of the nervous system: malformation. In: de Lahunta A, Glass E, eds. Veterinary Neuroanatomy and Clinical Neurology. 3rd ed. Saunders\/Elsevier; 2008.","edition":"3"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.06.017"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.7589\/2012-08-206"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid3008.240411"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11020265"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-016-0657-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.22.12211-12221.2003"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-018-1125-0"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres\/2010011"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2021.10.016"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2004.05.013"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1177\/0300985819837723"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2008.03.008"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.22.03.0112"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2019.03.005"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638714528502"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(93)90070-N"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.4.2.180-184.1997"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1177\/0300985818798085"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2016.1249546"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-025-04560-y"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/379441a0"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-37.3.594"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0021"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/s44149-023-00107-6"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3801-3805.2003"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638719876303"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638720912229"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638720967381"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0230"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858261465442","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03009858261465442","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03009858261465442","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T07:01:07Z","timestamp":1793516467000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03009858261465442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1177\/03009858261465442"],"URL":"https:\/\/doi.org\/10.1177\/03009858261465442","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]}}}