{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T18:54:38Z","timestamp":1777920878378,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pathol"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:p>We investigated the expression of interleukin-8 (IL-8) in pneumonic pasteurellosis of cattle because neutrophils are important mediators of tissue injury in this disease and because IL-8 is a major neutrophil chemoattractant in other species. We also compared IL-8 expression in bacterial and viral pneumonia, since the latter lacks the severe neutrophil exudation typical of pneumonic pasteurellosis. IL-8 expression was assessed by northern analysis, in situ hybridization, enzyme-linked immunosorbent assay, and in vivo bioassay. IL-8 mRNA expression was elevated dramatically in lesions of pneumonic pasteurellosis compared to unaffected lung from the same calves. In situ hybridization revealed intense expression of IL-8 mRNA in alveolar macrophages and neutrophils and milder expression in bronchiolar and alveolar epithelium, interstitial cells, and pleural mesothelium. Bronchoalveolar lavage fluid from lesional lung contained 16.06 \u00b1 4.00 ng\/ml IL-8, whereas those from nonlesional and normal lung contained 0.34 \u00b1 0.11 and 0.01 \u00b1 0.002 ng\/ml, respectively. We detected IL-8 expression at only minimal levels in bovine respiratory syncytial viral pneumonia. Lung extracts from lesions of pneumonic pasteurellosis induced vigorous neutrophil infiltration following injection into bovine skin, and 89% depletion of IL-8 from the extract reduced this neutrophil influx by 60%. These results demonstrate consistent upregulation of IL-8 expression in lesions of pneumonic pasteurellosis, implying a role for IL-8 in the ongoing recruitment of neutrophils to established lesions of pneumonic pasteurellosis. Because neutrophil-mediated tissue injury is critical to the pathogenesis of pneumonic pasteurellosis, these data suggest that neutralization of IL-8 activity could ameliorate the severe clinical signs and lesions of this disease.<\/jats:p>","DOI":"10.1177\/030098589803500206","type":"journal-article","created":{"date-parts":[[2010,1,22]],"date-time":"2010-01-22T22:24:55Z","timestamp":1264199095000},"page":"124-131","source":"Crossref","is-referenced-by-count":61,"title":["Expression of the Neutrophil Chemoattractant Interleukin-8 in the Lesions of Bovine Pneumonic Pasteurellosis"],"prefix":"10.1177","volume":"35","author":[{"given":"J. L.","family":"Caswell","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. M.","family":"Middleton","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. D.","family":"Sorden","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1998,3,1]]},"reference":[{"key":"bibr1-030098589803500206","first-page":"1382","volume":"145","author":"Allmann-Iselin I","year":"1994","journal-title":"Am J Pathol"},{"key":"bibr2-030098589803500206","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.2460\/ajvr.1981.42.08.1383","volume":"42","author":"Berggren HA","year":"1981","journal-title":"Am J Vet Res"},{"key":"bibr3-030098589803500206","first-page":"2960","volume":"152","author":"Broaddus VC","year":"1994","journal-title":"Immunol"},{"key":"bibr4-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/146.4.825"},{"key":"bibr5-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.9.3126-3133.1991"},{"key":"bibr6-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(95)05520-7"},{"key":"bibr7-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1038\/346274a0"},{"key":"bibr8-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.6.2027"},{"key":"bibr9-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(94)90001-9"},{"key":"bibr10-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/2.6.479"},{"key":"bibr11-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(92)90034-N"},{"issue":"1","key":"bibr12-030098589803500206","doi-asserted-by":"crossref","first-page":"A35","DOI":"10.1038\/icb.1997.6","volume":"75","author":"Matsumisha K","year":"1997","journal-title":"Immunol Cell Biol"},{"key":"bibr13-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1996.0019"},{"key":"bibr14-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(93)90058-C"},{"key":"bibr15-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1021\/bi00089a037"},{"key":"bibr16-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1986.60.2.532"},{"key":"bibr17-030098589803500206","first-page":"689","volume":"72","author":"Rogivue C","year":"1995","journal-title":"Lab Invest"},{"key":"bibr18-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1038\/365654a0"},{"key":"bibr19-030098589803500206","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.2460\/ajvr.1985.46.11.2253","volume":"46","author":"Slocombe RF","year":"1985","journal-title":"Am J Vet Res"},{"key":"bibr20-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1994.59"},{"key":"bibr21-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.59.1.24"},{"key":"bibr22-030098589803500206","doi-asserted-by":"crossref","first-page":"337","DOI":"10.2460\/ajvr.1991.52.02.337","volume":"52","author":"Weiss DJ","year":"1991","journal-title":"Am J Vet Res"},{"key":"bibr23-030098589803500206","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1992.tb00980.x"},{"key":"bibr24-030098589803500206","first-page":"225","volume":"46","author":"Yates WDG","year":"1982","journal-title":"Can J Comp Med"}],"container-title":["Veterinary Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/030098589803500206","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/030098589803500206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T19:38:07Z","timestamp":1777664287000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/030098589803500206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["10.1177\/030098589803500206"],"URL":"https:\/\/doi.org\/10.1177\/030098589803500206","relation":{},"ISSN":["0300-9858","1544-2217"],"issn-type":[{"value":"0300-9858","type":"print"},{"value":"1544-2217","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3]]}}}