{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:23:23Z","timestamp":1777490603806,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2014,2,21]],"date-time":"2014-02-21T00:00:00Z","timestamp":1392940800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cephalalgia"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>In healthy human volunteers and in migraineurs, pituitary adenylate cyclase-activating polypeptide-38 (PACAP-38) infusion caused sustained vasodilation of the middle meningeal artery (MMA) and an immediate as well as a delayed headache. All the study subjects experienced facial flushing. Mast cells (MCs) might have a role in the long-lasting effect of PACAP-38 infusion. We hypothesized that in mast cell-depleted (MCD) rats the vascular responses to PACAP-38 would be lesser than in control rats because of a lack of vasodilatory products released during MC degranulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      MCs were depleted by chronic treatment with compound 48\/80. The effect of 20 minutes' intravenous (i.v.) infusion of calcitonin gene-related peptide (CGRP), PACAP-38, PACAP(6-38) (PAC-1 receptor antagonist) and PACAP-27 on the diameter of the MMA and on mean arterial blood pressure (MABP) in control and MCD rats was recorded by using the genuine closed-cranial window (CCW) model. Vasoactive intestinal polypeptide (VIP) infusion was given only in control rats. A combination of the histamine H\n                      <jats:sub>1<\/jats:sub>\n                      receptor antagonist mepyramine (4\u2009mg\u2009kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      i.v.) and the H\n                      <jats:sub>2<\/jats:sub>\n                      receptor antagonist famotidine (1\u2009mg\u2009kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      i.v.) was given 10 minutes prior to PACAP-38 infusion. Increasing doses of PACAP-38, PACAP-27 and VIP were infused through the intracarotid artery (i.c.) in control and MCD rats to see the direct effects of these peptides on MMA diameter change.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      There was no significant change in CGRP-induced MMA diameter increase in control and MCD rats, and the dilated MMA immediately returned back to baseline after stopping the infusion. The delayed MMA dilation induced by PACAP-38 was abolished in MCD and antihistamine (AH)-pretreated rats. Compared to PACAP-38, the PACAP-27 i.v. infusion gave smaller peak dilation of MMA in control rats. In MCD rats, PACAP-27 did not induce any significant dilation. VIP i.v. infusion reduced MABP but did not dilate MMA significantly. PACAP(6-38), which is a potent MC degranulator, also gave a significant delayed dilation of MMA. PACAP-38 i.c. responses (direct receptor mediated response) were not affected by MC depletion. Only the maximum response (% E\n                      <jats:sub>max<\/jats:sub>\n                      ) value of PACAP-27 (i.c.) was significantly lower in MCD rats compared to control rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The delayed MMA dilatory responses to PACAP-38 infusion were attenuated in MCD and AH-pretreated rats, indicating a role of the MC mediator-histamine in PACAP-38-induced delayed dilation of MA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0333102414523846","type":"journal-article","created":{"date-parts":[[2014,2,22]],"date-time":"2014-02-22T01:09:23Z","timestamp":1393031363000},"page":"877-886","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":54,"title":["PACAP-38 infusion causes sustained vasodilation of the middle meningeal artery in the rat: Possible involvement of mast cells"],"prefix":"10.1177","volume":"34","author":[{"given":"Deepak K","family":"Bhatt","sequence":"first","affiliation":[{"name":"Danish Headache Center, Department of Neurology, Glostrup Hospital, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saurabh","family":"Gupta","sequence":"additional","affiliation":[{"name":"Danish Headache Center, Department of Neurology, Glostrup Hospital, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes","family":"Olesen","sequence":"additional","affiliation":[{"name":"Danish Headache Center, Department of Neurology, Glostrup Hospital, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inger","family":"Jansen-Olesen","sequence":"additional","affiliation":[{"name":"Danish Headache Center, Department of Neurology, Glostrup Hospital, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,2,21]]},"reference":[{"key":"bibr1-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61729-2"},{"key":"bibr2-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awn307"},{"key":"bibr3-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1177\/0333102411431333"},{"key":"bibr4-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-2982.2007.01497.x"},{"key":"bibr5-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1177\/0333102413483931"},{"key":"bibr6-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-2982.2008.01807.x"},{"key":"bibr7-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2007.05.002"},{"key":"bibr8-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2004.11.006"},{"key":"bibr9-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1177\/0333102412439354"},{"key":"bibr10-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-2982.2009.01888.x"},{"key":"bibr11-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1989.62.1.48"},{"key":"bibr12-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)03485-6"},{"key":"bibr13-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1177\/0333102412466967"},{"key":"bibr14-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-012-9851-0"},{"key":"bibr15-0333102414523846","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2007.02705.x"},{"key":"bibr16-0333102414523846","first-page":"1044","volume":"44","author":"Mori 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