{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:38:51Z","timestamp":1790707131611,"version":"4.1.0"},"reference-count":37,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T00:00:00Z","timestamp":1500854400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cephalalgia"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>A common characteristic of migraine-inducing substances is that they cause headache and no pain in other areas of the body. Few studies have compared pain mechanisms in the trigeminal and spinal systems and, so far, no major differences have been noted. We compared signalling molecules in the trigeminal and spinothalamic system after infusion of the migraine-provoking substance glyceryltrinitrate.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>A catheter was placed in the femoral vein of rats and one week later glyceryltrinitrate 4\u2009\u00b5g\/kg\/min was infused for 20\u2009min. Protein expression in the dura mater, trigeminal ganglion, nucleus caudalis, dorsal root ganglion and the dorsal horn of the thoracic spinal cord was analysed at different time points using western blotting and immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Glyceryltrinitrate caused a threefold increase in expression of phosphorylated extracellular signal-regulated kinases at 30\u2009min in the dura mater and nucleus caudalis ( P\u2009&lt;\u20090.05) and at 2\u2009h in the trigeminal ganglion with very few expressions in the dorsal root ganglion. In the nucleus caudalis, expression of phosphorylated extracellular signal-regulated kinases and Cam KII increased 2.6-fold and 3.2-fold, respectively, at 2\u2009h after glycerytrinitrate infusion ( P\u2009&lt;\u20090.01). p-CREB\/ATF-1 upregulation was observed only at 30\u2009min ( P\u2009&lt;\u20090.05) in the nucleus caudalis. None of these markers showed increased expression in the regions of thoracic spinal cord dorsal horn.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The dura, trigeminal ganglion and nucleus caudalis are activated shortly after glycerytrinitrate infusion with long-lasting expression of phosphorylated extracellular signal-regulated kinases observed in the nucleus caudalis. These activations were not observed at the spinal level.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0333102417722511","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T02:36:16Z","timestamp":1500950176000},"page":"1057-1070","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Selective cephalic upregulation of p-ERK, CamKII and p-CREB in response to glyceryl trinitrate infusion"],"prefix":"10.1177","volume":"38","author":[{"given":"Roshni","family":"Ramachandran","sequence":"first","affiliation":[{"name":"Danish Headache Centre, Department of Neurology, Glostrup Research Institute, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Glostrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Hougaard","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Danish Headache Centre, Department of Neurology, Glostrup Research Institute, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Glostrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipak Vasantrao","family":"Amrutkar","sequence":"additional","affiliation":[{"name":"Danish Headache Centre, Department of Neurology, Glostrup Research Institute, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Glostrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Petersen","sequence":"additional","affiliation":[{"name":"Danish Headache Centre, Department of Neurology, Glostrup Research Institute, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Glostrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie Mie","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Danish Headache Centre, Department of Neurology, Glostrup Research Institute, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Glostrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Hay-Schmidt","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, Panum Institute, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes","family":"Olesen","sequence":"additional","affiliation":[{"name":"Danish Headache Centre, Department of Neurology, Glostrup Research Institute, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Glostrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inger","family":"Jansen-Olesen","sequence":"additional","affiliation":[{"name":"Danish Headache Centre, Department of Neurology, Glostrup Research Institute, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Glostrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,7,24]]},"reference":[{"key":"bibr1-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.1994.tb00053.x"},{"key":"bibr2-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(89)90067-5"},{"key":"bibr3-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1177\/0333102411430855"},{"key":"bibr4-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1177\/0333102413502735"},{"key":"bibr5-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00029.2006"},{"key":"bibr6-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2128-08.2008"},{"key":"bibr7-0333102417722511","doi-asserted-by":"publisher","DOI":"10.3389\/neuro.01.024.2009"},{"key":"bibr8-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awg009"},{"key":"bibr9-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200209000-00010"},{"key":"bibr10-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2004.01412.x"},{"key":"bibr11-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007631200"},{"key":"bibr12-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.2000.0360"},{"key":"bibr13-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-11-04337.1999"},{"key":"bibr14-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203443"},{"key":"bibr15-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-02-00478.2002"},{"key":"bibr16-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.110601.135410"},{"key":"bibr17-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.06.048"},{"key":"bibr18-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.132167"},{"key":"bibr19-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2014.03.021"},{"key":"bibr20-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2010.08.050"},{"key":"bibr21-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1007\/s11916-012-0255-1"},{"key":"bibr22-0333102417722511","doi-asserted-by":"publisher","DOI":"10.2174\/1876386300902010011"},{"key":"bibr23-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-17-07737.2002"},{"key":"bibr24-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1177\/0333102415574846"},{"key":"bibr25-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23873"},{"key":"bibr26-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp168"},{"key":"bibr27-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-22-08842.2001"},{"key":"bibr28-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0374-11.2011"},{"key":"bibr29-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.31.060407.125631"},{"key":"bibr30-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2003.09.028"},{"key":"bibr31-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00693.2012"},{"key":"bibr32-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1021\/mp100029t"},{"key":"bibr33-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1126\/science.6166046"},{"key":"bibr34-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1074"},{"key":"bibr35-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200009280-00008"},{"key":"bibr36-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00075-1"},{"key":"bibr37-0333102417722511","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(02)00774-8"}],"container-title":["Cephalalgia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0333102417722511","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0333102417722511","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0333102417722511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:47:34Z","timestamp":1777387654000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0333102417722511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,24]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1177\/0333102417722511"],"URL":"https:\/\/doi.org\/10.1177\/0333102417722511","relation":{},"ISSN":["0333-1024","1468-2982"],"iss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