{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:04:46Z","timestamp":1791515086372,"version":"4.3.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2019,5,19]],"date-time":"2019-05-19T00:00:00Z","timestamp":1558224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"The Netherlands Organization for Scientific Research","award":["Vidi grant 917.113.349"],"award-info":[{"award-number":["Vidi grant 917.113.349"]}]},{"DOI":"10.13039\/100009997","name":"International Headache Society","doi-asserted-by":"publisher","award":["Post Doc fellowship"],"award-info":[{"award-number":["Post Doc fellowship"]}],"id":[{"id":"10.13039\/100009997","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["CONACyT, No 410778, No 409865"],"award-info":[{"award-number":["CONACyT, No 410778, No 409865"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cephalalgia"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The current understanding of mechanisms behind migraine pain has been greatly enhanced with the recent therapies targeting calcitonin gene-related peptide and its receptor. The clinical efficacy of calcitonin gene-related peptide-blocking drugs indicates that, at least in a considerable proportion of patients, calcitonin gene-related peptide is a key molecule in migraine pain. There are several receptors and molecular pathways that can affect the release of and response to calcitonin gene-related peptide. One of these could be purinergic receptors that are involved in nociception, but these are greatly understudied with respect to migraine.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We aimed to explore purinergic receptors as potential anti-migraine targets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used the human middle meningeal artery as a proxy for the trigeminal system to screen for possible anti-migraine candidates. The human findings were followed by intravital microscopy and calcitonin gene-related peptide release measurements in rodents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We show that the purinergic P2Y13 receptor fulfills all the features of a potential anti-migraine target. The P2Y13 receptor is expressed in both the human trigeminal ganglion and middle meningeal artery and activation of this receptor causes: a) middle meningeal artery contraction in vitro; b) reduced dural artery dilation following periarterial electrical stimulation in vivo and c) a reduction of CGRP release from both the dura and the trigeminal ganglion in situ. Furthermore, we show that P2X3 receptor activation of the trigeminal ganglion causes calcitonin gene-related peptide release and middle meningeal artery dilation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Both an agonist directed at the P2Y13 receptor and an antagonist of the P2X3 receptor seem to be viable potential anti-migraine therapies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0333102419851810","type":"journal-article","created":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:46:31Z","timestamp":1558313191000},"page":"1421-1434","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":37,"title":["Exploration of purinergic receptors as potential anti-migraine targets using established pre-clinical migraine models"],"prefix":"10.1177","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5182-8957","authenticated-orcid":false,"given":"Kristian A","family":"Haanes","sequence":"first","affiliation":[{"name":"Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands"},{"name":"Clinical Experimental Research Department, Copenhagen University Hospital, Rigshospitalet-Glostrup, Glostrup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2079-1156","authenticated-orcid":false,"given":"Alejandro","family":"Labastida-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank W","family":"Blixt","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2912-3632","authenticated-orcid":false,"given":"Eloisa","family":"Rubio-Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens M","family":"Dirven","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Erasmus Medical Center, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander HJ","family":"Danser","sequence":"additional","affiliation":[{"name":"Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Edvinsson","sequence":"additional","affiliation":[{"name":"Clinical Experimental Research Department, Copenhagen University Hospital, Rigshospitalet-Glostrup, Glostrup, Denmark"},{"name":"Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoinette","family":"MaassenVanDenBrink","sequence":"additional","affiliation":[{"name":"Division of Vascular Medicine and Pharmacology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,5,19]]},"reference":[{"key":"bibr1-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-018-0003-1"},{"key":"bibr2-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2012.01.005"},{"key":"bibr3-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410330109"},{"key":"bibr4-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1097\/WNF.0000000000000227"},{"key":"bibr5-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-6497-0"},{"key":"bibr6-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-015-9462-7"},{"key":"bibr7-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-015-9453-8"},{"key":"bibr8-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1186\/1129-2377-15-24"},{"key":"bibr9-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2005.06.029"},{"key":"bibr10-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0647-06.2006"},{"key":"bibr11-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.10201"},{"key":"bibr12-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2007.10.003"},{"key":"bibr13-0333102419851810","doi-asserted-by":"publisher","DOI":"10.1002\/wmts.62"},{"key":"bibr14-0333102419851810","doi-asserted-by":"crossref","unstructured":"Haanes KA and Edvinsson L. Pathophysiological mechanisms in nigraine and the identification of new therapeutic targets.\n                      CNS Drugs\n                      . Epub ahead of print 15 April 2019. 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