{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:07:20Z","timestamp":1787018840474,"version":"build-2736575974"},"reference-count":47,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82230085"],"award-info":[{"award-number":["82230085"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272572"],"award-info":[{"award-number":["82272572"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81974338"],"award-info":[{"award-number":["81974338"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019081","name":"Science and Technology Program of Hunan Province","doi-asserted-by":"publisher","award":["kh2102015"],"award-info":[{"award-number":["kh2102015"]}],"id":[{"id":"10.13039\/501100019081","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012476","name":"Fundamental Research Funds for Central Universities of the Central South University","doi-asserted-by":"publisher","award":["2022ZZTS0855"],"award-info":[{"award-number":["2022ZZTS0855"]}],"id":[{"id":"10.13039\/501100012476","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Sports Med"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Bone morphogenetic protein 2 (BMP2) is an appealing osteogenic and chondrogenic growth factor for promoting tendon-bone healing. Recently, it has been reported that soluble vascular endothelial growth factor (VEGF) receptor 1 (sVEGFR1) (a VEGF receptor antagonist) could enhance BMP2-induced bone repair and cartilage regeneration; thus, their combined application may represent a promising treatment to improve tendon-bone healing. Moreover, BMP2 could stimulate skeletal stem cell (SSC) expansion and formation, which is responsible for wounded tendon-bone interface repair. However, whether the codelivery of BMP2 and sVEGFR1 increases tendon enthesis injury\u2013activated SSCs better than does BMP2 alone needs further research.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To study the effect of BMP2 combined with sVEGFR1 on tendon-bone healing and injury-activated SSC lineage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design:<\/jats:title>\n                    <jats:p>Controlled laboratory study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A total of 128 C57BL\/6 mice that underwent unilateral supraspinatus tendon detachment and repair were randomly assigned to 4 groups: (1) untreated control group; (2) hydrogel group, which received a local injection of the blank hydrogel at the injured site; (3) BMP2 group, which received an injection of hydrogel with BMP2; and (4) BMP2 with sVEGFR1 group, which received an injection of hydrogel with BMP2 and sVEGFR1. Histology, micro\u2013computed tomography, and biomechanical tests were conducted to evaluate tendon-bone healing at 4 and 8 weeks after surgery. In addition, flow cytometry was performed to detect the proportion of SSCs and their downstream differentiated subtypes, including bone, cartilage, and stromal progenitors; osteoprogenitors; and pro-chondrogenic progenitors within supraspinatus tendon enthesis at 1 week postoperatively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The repaired interface in BMP2 with sVEGFR1 group showed a significantly improved collagen fiber continuity, increased fibrocartilage, greater newly formed bone, and elevated mechanical properties compared with the other 3 groups. There were more SSCs; bone, cartilage, and stromal progenitors; osteoprogenitors; and pro-chondrogenic progenitors in the BMP2 with sVEGFR1 group than that in the other groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Our study suggests that the combined delivery of BMP2 and sVEGFR1 could promote tendon-bone healing and stimulate the expansion of SSCs and their downstream progeny within the injured tendon-bone interface.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance:<\/jats:title>\n                    <jats:p>Combining BMP2 with sVEGFR1 may be a good clinical treatment for wounded tendon enthesis healing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03635465231225244","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T07:27:53Z","timestamp":1707982073000},"page":"779-790","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["A Combined Treatment of BMP2 and Soluble VEGFR1 for the Enhancement of Tendon-Bone Healing by Regulating Injury-Activated Skeletal Stem Cell Lineage"],"prefix":"10.1177","volume":"52","author":[{"given":"Linfeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China"},{"name":"Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China"},{"name":"Hunan Engineering Research Center of Sports and Health, Changsha, China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyang","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China"},{"name":"Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China"},{"name":"Hunan Engineering Research Center of Sports and Health, Changsha, China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China"},{"name":"Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China"},{"name":"Hunan Engineering Research Center of Sports and Health, Changsha, China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changbiao","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China"},{"name":"Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China"},{"name":"Hunan Engineering Research Center of Sports and Health, Changsha, China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China"},{"name":"Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China"},{"name":"Hunan Engineering Research Center of Sports and Health, Changsha, China"},{"name":"Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, China. Linfeng Wang and Liyang Wan contributed equally to this study"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daqi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China"},{"name":"Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China"},{"name":"Hunan Engineering Research Center of Sports and Health, Changsha, China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China"},{"name":"Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China"},{"name":"Hunan Engineering Research Center of Sports 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