{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:21:11Z","timestamp":1787318471693,"version":"build-2736575974"},"reference-count":37,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Sports Med"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Mesenchymal stromal cell (MSC) techniques represent a promising method to enhance the surgical repair of rotator cuff tears. To eliminate the resource-intensive process of cell isolation and culture expansion, a method to recruit endogenous MSCs was investigated in an established rat model of rotator cuff repair.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis:<\/jats:title>\n                    <jats:p>MSCs can be pharmacologically mobilized from the peripheral blood and recruited to the operative rotator cuff to enhance tendon-bone healing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design:<\/jats:title>\n                    <jats:p>Controlled laboratory study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      The rat model of supraspinatus tendon detachment and acute surgical repair was used to compare the ability of 3 different chemokines (SDF-1\u03b2, MIP-3\u03b1, and MCP-1) to recruit optically labeled MSCs to the operative shoulder from circulation. Additional experimentation was undertaken to assess the effects of pharmacological MSC mobilization using a combination of a \u03b2\n                      <jats:sub>3<\/jats:sub>\n                      adrenoreceptor agonist (BRL37344) and a CXCR4 antagonist (AMD3100) on chemokine-directed recruitment to the shoulder. Finally, the effects of this therapeutic strategy on tendon-bone healing were assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      MCP-1\u2013loaded hydrogels recruited the greatest number of MSCs from circulation. MCP-1\u2013driven MSC recruitment was significantly enhanced by a regimen of subcutaneous BRL37344 and AMD3100. Postmortem micro\u2013computed tomography imaging performed at a 6-week endpoint revealed that local MCP-1 delivery was associated with significant reductions in trabecular spacing and apparent mineral density, and a significant increase in trabecular number, while pharmacological MSC mobilization had no significant effects. MCP-1 delivery was associated with a lower tendon cross-sectional area and a significant increase in percent relaxation (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = .006). Pharmacological MSC mobilization was associated with significantly increased peak stress (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = .039), significantly increased elastic modulus (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = .037), and a nonsignificant increase in both equilibrium stress (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = .057) and ultimate stress (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = .058). Local MCP-1 delivery was associated with significant improvements in tenocyte morphology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Endogenous MSCs can be pharmacologically mobilized into peripheral blood and recruited to the site of rotator cuff repair via local delivery of MCP-1. This therapeutic strategy was associated with improvements in the static and dynamic mechanical properties of the tendon-bone interface.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance:<\/jats:title>\n                    <jats:p>The healing of rotator cuff repairs represents an ongoing clinical challenge in orthopaedic surgery. This study demonstrates a method to use endogenous MSCs to enhance healing of the rotator cuff.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03635465251341439","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T07:22:08Z","timestamp":1748589728000},"page":"1806-1816","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Pharmacologic Mobilization and Chemokine-Directed Recruitment of Mesenchymal Stromal Cells to the Surgically Repaired Rotator Cuff"],"prefix":"10.1177","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8257-5673","authenticated-orcid":false,"given":"Kevin C.","family":"Baker","sequence":"first","affiliation":[{"name":"Henry Ford Health + Michigan State University Health Sciences, Detroit, Michigan, USA"},{"name":"Bone &amp; Joint Center, Department of Orthopaedic Surgery, Henry Ford Health, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mackenzie","family":"Fleischer","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Corewell Health William Beaumont University Hospital, Royal Oak, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1262-0613","authenticated-orcid":false,"given":"Michael D.","family":"Newton","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Galasso","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Corewell Health William Beaumont University Hospital, Royal Oak, Michigan, USA"},{"name":"North Idaho Orthopedics &amp; Sports Medicine, Ponderay, Idaho, 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