{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T15:55:33Z","timestamp":1793030133922,"version":"4.5.0"},"reference-count":53,"publisher":"SAGE Publications","issue":"13","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Sports Med"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>To date, no targeted pharmacological agents have been clinically available specifically for postoperative management after anterior cruciate ligament reconstruction (ACLR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To elucidate the role of macrophage senescence in the tendon-bone interface microenvironment and assess whether targeting CD14 can mitigate senescence, thereby enhancing tendon-bone healing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design:<\/jats:title>\n                    <jats:p>Controlled laboratory study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A murine ACLR model was used to evaluate the tendon-bone healing. Healing was assessed 8 weeks postsurgery through histological staining, micro\u2013computed tomography analysis of neoplastic bone formation within bone tunnels, and biomechanical testing of tendon grafts. Cellular senescence was evaluated using \u03b2-galactosidase staining, while immunofluorescence and immunohistochemistry were used to analyze protein expression levels. Macrophage heterogeneity at the tendon-bone interface was assessed via t-distributed stochastic neighbor embedding projection, and senescent macrophage characteristics were investigated using CellChat, KEGG, and GO analyses. Alkaline phosphatase and Alizarin Red S staining were used to evaluate osteogenic differentiation of bone marrow mesenchymal stem cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Early inflammatory responses triggered by apoptotic cells at the tendon-bone interface resulted in macrophage senescence, activation of inflammatory pathways, increased secretion of pro-inflammatory factors, and elevated CD14 expression. Targeting CD14 reduced macrophage senescence and the inflammatory response at the tendon-bone interface, thereby increasing tendon-bone healing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The findings indicate that excessive inflammation within the tendon-bone interface microenvironment promotes macrophage senescence, thereby impairing tendon-bone healing. Targeting CD14 effectively prevents macrophage senescence, facilitating improved tendon-bone healing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance:<\/jats:title>\n                    <jats:p>Currently, targeted therapeutics to enhance tendon-bone healing post-ACLR are lacking in clinical practice. The findings demonstrate that microenvironmental inflammation leading to macrophage senescence is a critical factor contributing to impaired tendon-bone healing. CD14-targeted therapy may inhibit macrophage senescence, accelerate tendon-bone healing, and offer significant translational potential for clinical application.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03635465251383161","type":"journal-article","created":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T04:36:27Z","timestamp":1760762187000},"page":"3192-3206","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Targeting CD14 to Inhibit Macrophage Senescence and Regulate the Microenvironment for Enhanced Tendon-Bone Healing"],"prefix":"10.1177","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9490-4577","authenticated-orcid":false,"given":"Juncheng","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Sports Medicine, Center for Orthopedic Surgery, The Third Affiliated Hospital, Southern Medical University, Guangzhou, China"},{"name":"Guangdong 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