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While much is known about the function of the agr as a quorum sensing system that regulates virulence, not much is known about TRAP. TRAP is a 167-kDa protein that is highly conserved among staphylococci and is involved in DNA protection from stress. TRAP is membrane-associated but does not have a transmembrane domain, and thus it may be bound to the membrane through other proteins. To search for these proteins, protein-protein interaction studies were carried out using a bacterial two-hybrid system, and OpuCA was discovered as a TRAP-binding protein. OpuCA is an ATP binding-cytoplasmic (ABC) domain of an OpuC ABC transporter. S. aureus OpuC\u2013 mutant strain was constructed and shown to be less tolerant to salt stress, and was defective in choline uptake. OpuC\u2013 cells were less pathogenic and showed reduced TRAP phosphorylation and agr activity, did not respond to RAP, and were defective in biofilm formation in vitro and in vivo. These results suggest that OpuC acts as a transporter and also plays a role in S. aureus pathogenesis.<\/jats:p>","DOI":"10.1177\/039139880903200909","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T02:04:05Z","timestamp":1515722645000},"page":"600-610","source":"Crossref","is-referenced-by-count":23,"title":["OpuC \u2013 an ABC Transporter that is Associated with\n                    <i>Staphylococcus Aureus<\/i>\n                    Pathogenesis"],"prefix":"10.1177","volume":"32","author":[{"given":"Madanahally D.","family":"Kiran","sequence":"first","affiliation":[{"name":"Tufts University, Cummings School of Veterinary Medicine, Department of Biomedical Sciences, North Grafton, MA - USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna E.","family":"Akiyoshi","sequence":"additional","affiliation":[{"name":"Tufts University, Cummings School of Veterinary Medicine, Department of Biomedical Sciences, North Grafton, MA - 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