{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T05:01:04Z","timestamp":1777784464113,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2009,9,1]],"date-time":"2009-09-01T00:00:00Z","timestamp":1251763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Artif Organs"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:p>This report focuses on the molecular characterization of a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection and recognized retrospectively as a carrier of the Panton-Valentine leukocidin gene. The stored microbiological isolate, which belonged to the strain collection of the Research Unit on Implant Infections of the Rizzoli Orthopaedic Institute, was retrieved for molecular analysis. Genotyping was carried out, revealing an interesting profile. In addition to the positivity for the Panton-Valentine toxin gene, the results indicated that the isolate belonged to the agr III group and was endowed with bbp and cna genes, both encoding for staphylococcal adhesins that bind bone proteins. The strain had the mecA gene for methicillin resistance, even though it was unable to resist any of the \u03b2-lactam or other antibiotics. Its gene configuration matched that of other community-acquired methicillin-resistant and methicillin-susceptible Staphylococcus aureus (CA-MRSA and CA-MSSA) strains which have recently been reported worldwide. As far as we know, this is the first report on a PVL-positive S. aureus strain associated with an orthopedic implant (knee arthroprosthesis) infection.<\/jats:p>","DOI":"10.1177\/039139880903200912","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T02:04:05Z","timestamp":1515722645000},"page":"630-634","source":"Crossref","is-referenced-by-count":6,"title":["Panton-Valentine Leukocidin Gene Detected in a\n                    <i>Staphylococcus Aureus<\/i>\n                    Strain Isolated from a Knee Arthroprosthesis Infection"],"prefix":"10.1177","volume":"32","author":[{"given":"Lucio","family":"Montanaro","sequence":"first","affiliation":[{"name":"Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy"},{"name":"Experimental Pathology Department, University of Bologna, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucilla","family":"Baldassarri","sequence":"additional","affiliation":[{"name":"Respiratory and Systemic Disease Unit, Department of Infectious, Parasitic and Immuno-mediated Diseases, National Health Institute, Rome - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tolmino","family":"Corazzari","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Physics, University of Modena and Reggio Emilia, Modena - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Creti","sequence":"additional","affiliation":[{"name":"Respiratory and Systemic Disease Unit, Department of Infectious, Parasitic and Immuno-mediated Diseases, National Health Institute, Rome - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Ravaioli","sequence":"additional","affiliation":[{"name":"Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy"},{"name":"Experimental Pathology Department, University of Bologna, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"Cangini","sequence":"additional","affiliation":[{"name":"Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy"},{"name":"Experimental Pathology Department, University of Bologna, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valter","family":"Pirini","sequence":"additional","affiliation":[{"name":"Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Maso","sequence":"additional","affiliation":[{"name":"Microbiological Analysis Section of the Musculoskeletal Tissue Bank, Rizzoli Orthopedic Institute, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. ELENA","family":"Donati","sequence":"additional","affiliation":[{"name":"Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy"},{"name":"Currently at the Microbiological Analysis Section of the Musculoskeletal Tissue Bank, Rizzoli Orthopedic Institute, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Pegreffi","sequence":"additional","affiliation":[{"name":"Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livia","family":"Visai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Pavia, Pavia - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Campoccia","sequence":"additional","affiliation":[{"name":"Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietro","family":"Speziale","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Pavia, Pavia - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Renata","family":"Arciola","sequence":"additional","affiliation":[{"name":"Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna - Italy"},{"name":"Experimental Pathology Department, University of Bologna, Bologna - Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,1,10]]},"reference":[{"key":"bibr1-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1177\/039139880602900412"},{"key":"bibr2-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.07.006"},{"key":"bibr3-039139880903200912","volume-title":"Clinical and Laboratory Standards Institute (CLSI), Performance standards for antimicrobial susceptibility testing \u2013 approved standard: 18th informational supplement (M100-S18)","author":"Clinical and Laboratory Standards Institute,","year":"2008"},{"key":"bibr4-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4089-4094.2003"},{"key":"bibr5-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4465-4467.2003"},{"key":"bibr6-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.09.002"},{"key":"bibr7-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(99)00099-X"},{"key":"bibr8-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.06.038"},{"key":"bibr9-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1434-1439.2003"},{"key":"bibr10-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2004.03.009"},{"key":"bibr11-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1177\/039139880703000908"},{"key":"bibr12-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.06.038"},{"key":"bibr13-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.03.049"},{"key":"bibr14-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1177\/039139880502801106"},{"key":"bibr15-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1177\/039139880502801103"},{"key":"bibr16-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1177\/039139880602900404"},{"key":"bibr17-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1177\/039139880602900417"},{"key":"bibr18-039139880903200912","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s10156-009-0667-Y","volume":"15","author":"Reva I.","year":"2009","journal-title":"J Infect Chemother"},{"key":"bibr19-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00966-08"},{"key":"bibr20-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.30.7.1685-1691.1992"},{"key":"bibr21-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-113"},{"key":"bibr22-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2004.apm11211-1208.x"},{"key":"bibr23-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1086\/511033"},{"key":"bibr24-039139880903200912","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.3.4.453"},{"key":"bibr25-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.03.007"},{"key":"bibr26-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-693X(07)80038-8"},{"key":"bibr27-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-693X(07)80043-1"},{"key":"bibr28-039139880903200912","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200969060-00004"},{"key":"bibr29-039139880903200912","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2008.10.008"}],"container-title":["The International Journal of Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/039139880903200912","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/039139880903200912","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:40:52Z","timestamp":1777491652000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/039139880903200912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9]]},"references-count":29,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["10.1177\/039139880903200912"],"URL":"https:\/\/doi.org\/10.1177\/039139880903200912","relation":{},"ISSN":["0391-3988","1724-6040"],"issn-type":[{"value":"0391-3988","type":"print"},{"value":"1724-6040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9]]}}}