{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:08:02Z","timestamp":1791094082825,"version":"4.1.0"},"reference-count":24,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Artif Organs"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To test the time-dependent effects of rifampicin on established biofilms of Staphylococcus epidermidis isolated from patients with cardiac implant infections and catheter-related bacteremia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Biofilms were grown in microtiter plates for 24 hours, dyed and stained with crystal violet. The mean optical density (OD) was used for quantification. The OD ratio (OD\n                      <jats:sub>r<\/jats:sub>\n                      = OD of the treated biofilm\/OD of the untreated biofilm) was used to measure changes in the thickness of the biofilms over the time. Biofilms were incubated with rifampicin (0.6 mg\/mL) for 1, 5, 15, 30 and 60 minutes. Unstained biofilms were sonicated and plated on Columbia agar for time-kill curves.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The incubation of the biofilms with rifampicin led to a significant reduction of the OD of the biofilms within 1 minute (OD\n                      <jats:sub>r<\/jats:sub>\n                      baseline: 1; OD\n                      <jats:sub>r<\/jats:sub>\n                      1 min: 0.333 \u00b1 0.131) (p&lt;0.001). With regard to bacterial killing, rifampicin reduced the mean log count, but viable bacteria were still grown from biofilms in 13 out of 28 isolates despite MIC values &lt; 0.01 mg\/L.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In conclusion, our results confirm that rifampicin at a concentration of 1.2 mg\/mL immediately reduces established biofilms formed by S. epidermidis although it is not bactericidal despite very low MICs at planktonic conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/039139881003300907","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T11:26:09Z","timestamp":1516793169000},"page":"621-626","source":"Crossref","is-referenced-by-count":14,"title":["Time-Dependent Effects of Rifampicin on Staphylococcal Biofilms"],"prefix":"10.1177","volume":"33","author":[{"given":"Klaus-Bernhard","family":"Gattringer","sequence":"first","affiliation":[{"name":"Department of Medicine I, Div. Infectious Diseases, Medical University of Vienna, Vienna - Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miranda","family":"Suchomel","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Institute of Hygiene, Medical University of Vienna, Vienna - Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Eder","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, Max-Planck-Institute of Colloids and Interfaces, Medical University of Vienna, Vienna - Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea M.","family":"Lassnigg","sequence":"additional","affiliation":[{"name":"Department of Anesthesia and General Intensive Care Medicine, Div. of Cardiothoracic Anesthesia, Medical University of Vienna, Vienna - Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Graninger","sequence":"additional","affiliation":[{"name":"Department of Medicine I, Div. Infectious Diseases, Medical University of Vienna, Vienna - Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Presterl","sequence":"additional","affiliation":[{"name":"Department of Medicine I, Div. Infectious Diseases, Medical University of Vienna, Vienna - Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,1,24]]},"reference":[{"key":"bibr1-039139881003300907","unstructured":"BaddourL.M., WilsonW.R. Infections of Prosthetic Valves and Other Cardiovascular Devices. In: MandellG., BenettJ., DolinR., eds. Practice and principles of infectious diseases. 6 ed. Philadelphia: Elsevier; 2005: 1022\u201344."},{"key":"bibr2-039139881003300907","unstructured":"BeckmannS.E., HendersonD.E. Infections Caused by Percutaneous Intravascular Devices. In: MandellG., BenettJ., DolinR., editors. Principles and practice of infectious diseases. 6 ed. Philadelphia: Elsevier; 2005; 3347\u201362."},{"key":"bibr3-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp0905029"},{"key":"bibr4-039139881003300907","first-page":"S62","volume":"57","author":"Peters G.","year":"1988","journal-title":"Br J Clin Pract (Suppl)"},{"key":"bibr5-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/36.4.681"},{"key":"bibr6-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"bibr7-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1080\/07853890802337045"},{"key":"bibr8-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1001\/jama.279.19.1537"},{"key":"bibr9-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70280-9"},{"key":"bibr10-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2005.05.017"},{"key":"bibr11-039139881003300907","volume-title":"Manual of Clinical Microbiology.","author":"Murray P.R.","year":"2003","edition":"8"},{"key":"bibr12-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1177\/039139880502801108"},{"key":"bibr13-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.22.6.996-1006.1985"},{"key":"bibr14-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02663.x"},{"key":"bibr15-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra035415"},{"key":"bibr16-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1001\/jama.279.19.1537"},{"key":"bibr17-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.1.55-61.2006"},{"key":"bibr18-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.3.900-903.2002"},{"key":"bibr19-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.30559"},{"key":"bibr20-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(86)90495-X"},{"key":"bibr21-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00833-8"},{"key":"bibr22-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm221"},{"key":"bibr23-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2001.01385.x"},{"key":"bibr24-039139881003300907","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-008-0778-7"}],"container-title":["The International Journal of Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/039139881003300907","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/039139881003300907","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:41:24Z","timestamp":1777491684000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/039139881003300907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":24,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1177\/039139881003300907"],"URL":"https:\/\/doi.org\/10.1177\/039139881003300907","relation":{},"ISSN":["0391-3988","1724-6040"],"issn-type":[{"value":"0391-3988","type":"print"},{"value":"1724-6040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]}}}