{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T04:59:46Z","timestamp":1777784386520,"version":"3.51.4"},"reference-count":26,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Ministerio del Poder Popular para Ciencia y Tecnolog\u00eda","award":["06910"],"award-info":[{"award-number":["06910"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Artif Organs"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Under certain clinical and experimental conditions hematopoiesis occurs in other site than bone marrow (BM), such as the liver. Here, we develop a 3D organoid that mimics several components of the hematopoietic niche present during liver extramedullary hematopoiesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>To evaluate the capacity of a 3D hematopoietic organoid (3D-HO) to function as a hematopoietic like-niche allowing for blood cell production outside of the BM<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>The 3D-HO is constituted by liver sinusoidal endothelial cells (LSEC) as the stromal component, BM isolated from 5-FU treated mice (FU-BMCs), collagen microspheres and plasma clot as scaffolds. The ability of the 3D-HO to support the survival and functionality of FU-BMCs was investigated by using confocal microscopy, histology analysis, flow cytometry, and clonogenic assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      After 15 and 30\u2009days, post-ectopic implantation, histological studies of the 3D-HO showed the presence of cells with myeloid and lymphoid lineage morphology. Flow cytometry analysis of these cells showed the presence of cells expressing hematopoietic stem progenitor cells (HSPC) (Sca-1\n                      <jats:sup>+<\/jats:sup>\n                      \/c-Kit\n                      <jats:sup>+<\/jats:sup>\n                      ), myeloid (Gr-1\n                      <jats:sup>+<\/jats:sup>\n                      ) and lymphoid (B220\n                      <jats:sup>+<\/jats:sup>\n                      and CD19\n                      <jats:sup>+<\/jats:sup>\n                      ) markers. Clonogenic assays showed that cells from the 3D-HO formed hematopoietic colonies. Expression of the Sry gene by cells from the 3D-HO, implanted for 30\u2009days in female mice, indicated that male donor cells persist in this model of extramedullary hematopoiesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The 3D-HO constitutes an extramedullary hematopoietic-like niche which supports the survival and functionality of FU-BMCs. It may constitute an efficient model for investigating, in vitro and in vivo, those factors that control hematopoiesis outside BM.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03913988221136144","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T04:50:29Z","timestamp":1667969429000},"page":"29-39","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["3D organoid modeling of extramedullary hematopoiesis"],"prefix":"10.1177","volume":"46","author":[{"given":"Eloisa","family":"Lara-Gonzalez","sequence":"first","affiliation":[{"name":"Unidad de Terapia Celular - Laboratorio de Patolog\u00eda Celular y Molecular, Centro de Medicina Regenerativa, Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Wittig","sequence":"additional","affiliation":[{"name":"Unidad de Terapia Celular - Laboratorio de Patolog\u00eda Celular y Molecular, Centro de Medicina Regenerativa, Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dylana","family":"Diaz-Solano","sequence":"additional","affiliation":[{"name":"Unidad de Terapia Celular - Laboratorio de Patolog\u00eda Celular y Molecular, Centro de Medicina Regenerativa, Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5597-5505","authenticated-orcid":false,"given":"Jose E.","family":"Cardier","sequence":"additional","affiliation":[{"name":"Unidad de Terapia Celular - Laboratorio de Patolog\u00eda Celular y Molecular, Centro de Medicina Regenerativa, Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"bibr1-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1089\/adt.2014.573"},{"key":"bibr2-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3647"},{"key":"bibr3-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.03.024"},{"key":"bibr4-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25905"},{"key":"bibr5-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2319"},{"key":"bibr6-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1038\/nature12984"},{"key":"bibr7-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0103-9"},{"key":"bibr8-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.53"},{"key":"bibr9-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.11.034"},{"key":"bibr10-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1002\/term.2507"},{"key":"bibr11-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2938"},{"key":"bibr12-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-020-03265-y"},{"key":"bibr13-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2019.01.024"},{"key":"bibr14-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1172\/JCI79328"},{"key":"bibr15-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510260122"},{"key":"bibr16-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.05.004"},{"key":"bibr17-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2014.04.017"},{"key":"bibr18-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2004.13.528"},{"key":"bibr19-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0300"},{"key":"bibr20-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24683"},{"key":"bibr21-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-018-9610-0"},{"key":"bibr22-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.029"},{"key":"bibr23-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.10.038"},{"key":"bibr24-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000259593.07661.1e"},{"key":"bibr25-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.06.017"},{"key":"bibr26-03913988221136144","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20172139"}],"container-title":["The International Journal of Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03913988221136144","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03913988221136144","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03913988221136144","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T19:40:41Z","timestamp":1777491641000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03913988221136144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,9]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1177\/03913988221136144"],"URL":"https:\/\/doi.org\/10.1177\/03913988221136144","relation":{},"ISSN":["0391-3988","1724-6040"],"issn-type":[{"value":"0391-3988","type":"print"},{"value":"1724-6040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,9]]}}}