{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T17:46:31Z","timestamp":1782582391467,"version":"3.54.5"},"reference-count":31,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","award":["223S322"],"award-info":[{"award-number":["223S322"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018703","name":"HORIZON EUROPE European Innovation Council","doi-asserted-by":"publisher","award":["101214454"],"award-info":[{"award-number":["101214454"]}],"id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Artif Organs"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:p>\n                    Conventional blood flow-loop systems, employing animal or rarely fresh human blood, used in in vitro testing of blood-contact devices fail to simulate the actual physiological conditions. This study aims to develop a metabolically sustainable blood flow loop system to be used in cardiovascular device testing, by integrating nutrition, oxygenation, and dialysis modules. Two flow loop systems with human blood were compared: (1) control circuit with oxygenation and (2) experimental circuit with the addition of nutrition, oxygenation, and dialysis modules. Blood was circulated for 12 and 48\u2009h, and hemolysis (NIH, pfHb, bilirubin, haptoglobin), blood gases (pH, glucose, lactate), inflammation (IL-8, TNF-\u03b1, C3a), coagulation (fibrinogen, TAT), platelet\/endothelial activation (\u03b2-TG, vWF), and ferritin levels were measured over time. While pH decrease, lactate increase, and inflammatory\/coagulation markers increased in the control group, these parameters were stable or decreased in the dialysis group. NIH was 77%\u201381% lower in dialysis, and vWF decreased 55%\u201373% in dialysis while increasing in control (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The improved flow loop system reduced hemolysis, inflammation, and coagulation by maintaining the metabolic balance and biochemical integrity of blood during long-term circulation. This approach has the potential to improve in vitro testing of blood-contacting medical devices.\n                  <\/jats:p>","DOI":"10.1177\/03913988261445012","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T06:28:41Z","timestamp":1781677721000},"page":"431-442","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Simulating physiological blood maintenance: A dialysis-integrated in vitro flow loop for realistic hemocompatibility evaluation of devices"],"prefix":"10.1177","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2652-7412","authenticated-orcid":false,"given":"Tansu","family":"G\u00f6lcez","sequence":"first","affiliation":[{"name":"Department of Biomedical Science and Engineering, Koc University, Istanbul, Turkey"},{"name":"Institute of Biomedical Engineering, Bogazici University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demet","family":"Kul","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine, Koc University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elif","family":"U\u011furel","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, Koc University, Istanbul, Turkey"},{"name":"Research Center for Translational Medicine (KUTTAM), Koc University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130rem","family":"G\u00fcnayd\u0131n","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Istanbul Medipol University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zlem","family":"Yal\u00e7\u0131n","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, Koc University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerem","family":"Pekkan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Nationwide Children\u2019s Hospital and The Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yahya","family":"Y\u0131ld\u0131z","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Reanimation, Istanbul Medipol University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,6,16]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2017-1155"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1603023113"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2125.2000.00194.x"},{"key":"e_1_3_2_5_2","volume-title":"An in vitro blood flow loop system for thrombogenicity evaluation of medical devices and biomaterials","author":"U.S. Food and Drug Administration","year":"2023","unstructured":"U.S. Food and Drug Administration. An in vitro blood flow loop system for thrombogenicity evaluation of medical devices and biomaterials. Silver Spring, MD: U.S. Food and Drug Administration, 2023."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/MAT.0000000000000958"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2018.00099"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13070793"},{"key":"e_1_3_2_9_2","first-page":"27","article-title":"Red blood cell storage lesion: causes and potential clinical consequences","volume":"17","author":"Yoshida T","year":"2019","unstructured":"Yoshida T, Prudent M, D\u2019Alessandro A. Red blood cell storage lesion: causes and potential clinical consequences. Blood Transfus 2019; 17: 27\u201352.","journal-title":"Blood Transfus"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.5301\/ijao.5000478"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/membranes11050313"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm14010140"},{"key":"e_1_3_2_13_2","unstructured":"ASTM International. ASTM F1841-97(2013): Standard practice for assessment of hemolytic properties of materials. West Conshohocken PA: ASTM International 2017."},{"key":"e_1_3_2_14_2","unstructured":"U.S. Food and Drug Administration. User manual for performing platelet and leukocyte count assays for in vitro hemocompatibility assessment of cardiovascular materials per ASTM F2888-19 standard. Silver Spring MD: U.S. Food and Drug Administration."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0251595"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3233\/BIR-140665"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.3233\/CH-221452"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-91855-2_11"},{"key":"e_1_3_2_19_2","volume-title":"10993-1: I. Biological evaluation of medical devices\u2014part 1: evaluation and testing within a risk management process","author":"International Organization for Standardization","year":"2018","unstructured":"International Organization for Standardization. 10993-1: I. Biological evaluation of medical devices\u2014part 1: evaluation and testing within a risk management process. Geneva: International Organization for Standardization, 2018."},{"key":"e_1_3_2_20_2","unstructured":"ASTM International. 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