{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:57:11Z","timestamp":1790499431452,"version":"4.1.0"},"reference-count":41,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Artif Organs"],"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Meniscal injury disrupts knee biomechanics and accelerates degeneration. Current treatments, including allografts, are limited by donor availability and biological constraints. Decellularized extracellular matrix (ECM) scaffolds offer a promising alternative. This study evaluated a three-dimensional (3D) decellularized bovine meniscal sponge scaffold and the effects of different crosslinking agents on its properties.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>An in vitro experimental study was conducted with four groups: human meniscus, non-crosslinked bovine scaffold, bovine scaffold treated with glutaraldehyde (GTA), and bovine scaffold treated with tannic acid. Scaffolds were characterized for morphology, ECM preservation, cytocompatibility, chemical properties, degradation, swelling, and mechanical performance using SEM, Masson\u2019s trichrome staining, MTT assay, FTIR, and mechanical testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The optimized decellularization protocol effectively removed cellular components while preserving ECM structure. All scaffolds exhibited a porous 3D architecture with maintained collagen content, especially in tannic acid-treated groups. Crosslinking significantly improved mechanical properties, with the highest compressive strength in the tannic acid 4-h group (2.51\u2009\u00b1\u20091.02\u2009MPa), followed by the GTA 24-h group (2.46\u2009\u00b1\u20091.15\u2009MPa). Crosslinked scaffolds also showed enhanced degradation resistance while maintaining adequate swelling capacity. All groups exhibited cell viability above 70%, indicating acceptable cytocompatibility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The 3D decellularized bovine meniscal scaffold shows strong potential as a meniscal graft substitute. Tannic acid crosslinking, particularly at 4\u2009h, provided the most balanced performance, supporting its potential for meniscus tissue engineering.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03913988261474356","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:57:58Z","timestamp":1790243878000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["3D decellularized bovine meniscal sponge scaffold as a graft candidate: Comparative in vitro study with standard grafts"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7211-6033","authenticated-orcid":false,"given":"Jifaldi Afrian Maharaja Dinda","family":"Sedar","sequence":"first","affiliation":[{"name":"Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Indonesia"},{"name":"Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7832-5695","authenticated-orcid":false,"given":"Dwikora Novembri","family":"Utomo","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Indonesia"},{"name":"Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5636-5100","authenticated-orcid":false,"given":"Prihartini","family":"Widiyanti","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Master Study Program, Department of Physics, Faculty of Science and Technology, Universitas Airlangga, Indonesia"},{"name":"Institute of Tropical Disease, Universitas Airlangga, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9384-897X","authenticated-orcid":false,"given":"Heri","family":"Suroto","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Indonesia"},{"name":"Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Indonesia"},{"name":"Cell and Tissue Bank-Regenerative Medicine Center, Dr. Soetomo General Academic Hospital, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-018-1031-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm14062020"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/life12040603"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.837554"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.03.074"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oto.2025.101180"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbad107"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/jfb13040240"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1155\/term\/3446671"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.37349\/ebmx.2025.101345"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.17691\/stm2022.14.3.07"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/gels10120767"},{"key":"e_1_3_2_14_2","first-page":"1","article-title":"Meniscal regenerative scaffolds based on biopolymers and polymers: recent status and applications","volume":"9","author":"Li H","year":"2021","unstructured":"Li H, Li P, Yang Z, et al. 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