{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T05:49:09Z","timestamp":1780120149698,"version":"3.54.0"},"reference-count":30,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Biol Markers"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Typically, the overexpressed keratin 7 (KRT7) is considered a validated therapeutic target and prognosis marker in bladder cancer. However, the crucial roles of KRT7 in the clinical prognosis and immune microenvironment in bladder cancer remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Initially, the expression levels of KRT7 in public databases were analyzed that is,Tumor Immune Estimation Resource (TIMER) 2.0 and Gene Expression Profiling Interactive Analysis (GEPIA). Further, the clinical tissue samples from patients (n\u2009=\u200910 pairs) were collected to confirm the expression trends of KRT7 and detected by immunohistochemistry (IHC) analysis. Meanwhile, the relationship between KRT7 and the prognosis of bladder cancer patients was analyzed by Kaplan\u2013Meier plotter estimation and Cox regression analysis. Finally, TIMER 2.0 and IHC staining analyses were performed to calculate the infiltration abundances of three kinds of immune cells in eligible bladder tumor samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The TIMER 2.0 and GEPIA datasets suggested the differences in the expression levels of KRT7 in tumors, in which KRT7 was significantly upregulated in bladder cancer. The KRT7 expression was closely associated with patients\u2019 gender, tumor histologic subtypes, T status, and American Joint Committee on Cancer stages. Notably, the increased KRT7 indicated poor overall survival and disease-free survival rates. Moreover, KRT7 expression could be responsible for immune infiltration in the cancer microenvironment of the bladder. Finally, the high expression level of KRT7 increased the presence of regulatory T cells (Tregs) but reduced the infiltration of CD8+ T and natural killer cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>KRT7 as a biomarker potentiated the prediction of bladder cancer prognosis and the immune microenvironment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03936155231224798","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T02:04:36Z","timestamp":1707271476000},"page":"158-167","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Clinical significance of KRT7 in bladder cancer prognosis"],"prefix":"10.1177","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6752-5673","authenticated-orcid":false,"given":"Jun","family":"Song","sequence":"first","affiliation":[{"name":"Department of Urology, The Third Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, Zhejiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology, The Third Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, Zhejiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongming","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology, The Third Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, Zhejiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Urology, The Third Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, Zhejiang, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunpei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Sanya People's Hospital, Sanya, Hainan, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Surgery, Sanya People's Hospital, Sanya, Hainan, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"key":"bibr1-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2005.07.062"},{"issue":"5","key":"bibr2-03936155231224798","first-page":"749","volume":"57","author":"Dobruch J","journal-title":"Medicina (Kaunas)"},{"key":"bibr3-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0000000000000697"},{"key":"bibr4-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.09.047"},{"key":"bibr5-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2015.06.045"},{"key":"bibr6-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00561-4"},{"key":"bibr7-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064404"},{"key":"bibr8-03936155231224798","doi-asserted-by":"publisher","DOI":"10.2147\/IJGM.S313584"},{"key":"bibr9-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-020-01362-0"},{"key":"bibr10-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05802-3"},{"key":"bibr11-03936155231224798","first-page":"1041","volume":"33","author":"Liu BL","year":"2019","journal-title":"J Biol Regul Homeost Agents"},{"key":"bibr12-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1186\/s12894-021-00838-z"},{"key":"bibr13-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe6474"},{"key":"bibr14-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.05.017"},{"key":"bibr15-03936155231224798","first-page":"07","volume":"04","author":"Wang S","year":"2023","journal-title":"Altern Ther Health Med"},{"key":"bibr16-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-022-01019-5"},{"key":"bibr17-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19933-0"},{"key":"bibr18-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-022-09983-6"},{"key":"bibr19-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7819-3-43"},{"key":"bibr20-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.003113"},{"key":"bibr21-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200603161"},{"key":"bibr22-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.6b00652"},{"key":"bibr23-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.23118"},{"key":"bibr24-03936155231224798","first-page":"12","volume":"01","author":"Xie R","year":"2022","journal-title":"Int J Mol Sci"},{"key":"bibr25-03936155231224798","doi-asserted-by":"publisher","DOI":"10.3892\/or.2020.7886"},{"key":"bibr26-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.25"},{"key":"bibr27-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1002\/iub.2468"},{"key":"bibr28-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106641"},{"key":"bibr29-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-96373-w"},{"key":"bibr30-03936155231224798","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14069"}],"container-title":["The International Journal of Biological Markers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03936155231224798","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03936155231224798","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03936155231224798","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:00:34Z","timestamp":1777492834000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03936155231224798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,6]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1177\/03936155231224798"],"URL":"https:\/\/doi.org\/10.1177\/03936155231224798","relation":{},"ISSN":["0393-6155","1724-6008"],"issn-type":[{"value":"0393-6155","type":"print"},{"value":"1724-6008","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,6]]}}}