{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:12:17Z","timestamp":1793146337377,"version":"4.5.0"},"reference-count":26,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Shandong Provincial Key Research and Development Project","award":["2019GSF108275"],"award-info":[{"award-number":["2019GSF108275"]}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"crossref","award":["2018M642671"],"award-info":[{"award-number":["2018M642671"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"crossref","award":["ZR2020MH315"],"award-info":[{"award-number":["ZR2020MH315"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Biol Markers"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>We aimed to exploit a urine exosomal long non-coding RNAs (lncRNAs) fingerprint to facilitate the early diagnosis of bladder cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Microarray differential expression profiling of lncRNAs was for the first time employed in urine exosomes from 10 non-muscle-invasive bladder cancer (NMIBC) patients and 10 healthy controls to screen out candidate exosomal lncRNA biomarkers, which were then verified by quantitative real-time polymerase chain reaction in three independent phases including bladder cancer cells, culture fluid and 200 NMIBC participants. Logistic regression was performed to construct a diagnostic model\u2014the diagnostic potency of which was assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The profile of three exosome-derived lncRNAs (CCDC148-AS1, XLOC_006419, and RP5-1148A21.3) was screened and further verified to be notably over-expressed in NMIBC patients and bladder cancer cell lines, and exhibited area under the receiver-operating characteristic curve values of 0.873, 0.825, and 0.834, respectively, in training, validation, and double-blind validation phases. The profile was superior to urinary cytology in discriminating NMIBC from healthy controls (\n                      <jats:italic toggle=\"yes\">P\u2009<\/jats:italic>\n                      &lt;\u20090.0001). A significant correlation existed between a higher level of CCDC148-AS1 and a higher tumor grade (\n                      <jats:italic toggle=\"yes\">P\u2009<\/jats:italic>\n                      &lt;\u20090.001), and up-regulated CCDC148-AS1 as well as XLOC_006419 were statistically related with tumor node metastasis stage (\n                      <jats:italic toggle=\"yes\">P\u2009<\/jats:italic>\n                      =\u20090.004 and\n                      <jats:italic toggle=\"yes\">P\u2009<\/jats:italic>\n                      =\u20090.031, respectively). These three identified lncRNAs were confirmed to originate from bladder cancer cells and be packaged within exosomes, thus staying sufficiently stable in urine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Tumor-originated urine exosomal lncRNAs, as fingerprint in NMIBC, exhibited satisfying clinical significance in early diagnosis of bladder cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03936155251317551","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T04:16:42Z","timestamp":1739420202000},"page":"24-34","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Urine exosomal lncRNAs as novel biomarkers for early diagnosis of bladder cancer based on microarray differential expression profiling"],"prefix":"10.1177","volume":"40","author":[{"given":"Jun","family":"Li","sequence":"first","affiliation":[{"name":"Linyi People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Linyi People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luning","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Linyi People's Hospital, Shandong Second Medical University, Linyi, Shandong Province 276003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Internal Medicine, Linyi People's Hospital, Shandong Second Medical University, Linyi, Shandong Province 276003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yisheng","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Urology, Linyi People's Hospital, Shandong Second Medical University, Linyi, Shandong Province 276003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongli","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Oncology, Linyi People's Hospital, Shandong Second Medical University, Linyi, Shandong Province 276003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4454-8997","authenticated-orcid":false,"given":"Jinfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Linyi People\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30512-8"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/1724600820926685"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/551S34a"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.008"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-018-0831-z"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.012"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.01.043"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-015-0181-x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-0975-5"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2017.10.040"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12094-023-03374-4"},{"key":"e_1_3_3_14_2","volume-title":"TNM classification of malignant tumors","author":"Sobin LH","year":"2009","unstructured":"Sobin LH, Gospodarowicz MK, Wittekind C. 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