{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T06:49:15Z","timestamp":1777790955539,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Science Research Foundation of Education Department of Yunnan Province","award":["2022J0716"],"award-info":[{"award-number":["2022J0716"]}]},{"name":"Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities","award":["202301BA070001-027"],"award-info":[{"award-number":["202301BA070001-027"]}]},{"name":"Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities","award":["202301BA070001-26"],"award-info":[{"award-number":["202301BA070001-26"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Biol Markers"],"abstract":"\n Purpose<\/jats:title>\n \n This study aimed to investigate KRAB-associated protein 1 (\n KAP1<\/jats:italic>\n ) expression in pleural mesothelioma (PM) and its impact on the biological behavior of the human pleural mesothelioma cell line MSTO-211H, providing a specific biomarker for the early clinical diagnosis of PM.\n <\/jats:p>\n <\/jats:sec>\n \n Patients and methods<\/jats:title>\n \n KAP1<\/jats:italic>\n expression levels in PM tissues were detected using immunohistochemistry. Lentivirus infection was used to construct MSTO-211H mesothelioma cell lines with stable\n KAP1<\/jats:italic>\n overexpression or knockdown, and the efficiency of\n KAP1<\/jats:italic>\n overexpression or knockdown was detected using qRT-PCR(quantitative Reverse Transcription PCR) and Western blotting. The effects of\n KAP1<\/jats:italic>\n overexpression and knockdown on MSTO-211H mesothelioma cell proliferation, migration, and invasion were detected using cell counting kit-8, plate colony formation, cell scratch, and transwell invasion assays, respectively. The effects of\n KAP1<\/jats:italic>\n overexpression and knockdown on the cell cycle, related cyclins, and apoptosis were detected using flow cytometry. Gene enrichment and correlation analysis of mesothelioma were performed using bioinformatics analysis.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n KAP1<\/jats:italic>\n was significantly overexpressed in PM tissues compared with normal pleural tissues (\n P<\/jats:italic>\n \u2009<\u20090.05). Compared to the control group,\n KAP1<\/jats:italic>\n overexpression in mesothelioma MSTO-211H cells significantly enhanced proliferation, migration, and invasion (\n P<\/jats:italic>\n \u2009<\u20090.05), without causing cell cycle arrest, and significantly increased the mRNA and protein levels of cyclin D1 and cyclin E (\n P<\/jats:italic>\n \u2009<\u20090.05), whereas the apoptosis rate did not significantly change (\n P<\/jats:italic>\n \u2009>\u20090.05). Conversely,\n KAP1<\/jats:italic>\n knockdown in mesothelioma MSTO-211H cells significantly inhibited their proliferation, migration, and invasion abilities (\n P<\/jats:italic>\n \u2009<\u20090.05), induced G0\/G1 phase arrest in the cell cycle, and significantly increased the apoptosis rate (\n P<\/jats:italic>\n \u2009<\u20090.05). Spearman correlation analysis revealed significant positive associations between\n KAP1<\/jats:italic>\n mRNA expression and\n TP53<\/jats:italic>\n and\n SHOX2<\/jats:italic>\n . Conversely,\n KAP1<\/jats:italic>\n expression was significantly negatively correlated with\n MTAP<\/jats:italic>\n and\n MSLN<\/jats:italic>\n . GSEA reveals\n KAP1<\/jats:italic>\n -associated enrichment of DNA repair, cell cycle, and proteostasis pathways in TCGA-MESO.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n KAP1<\/jats:italic>\n is highly expressed in PM and functions as an oncogene-like regulator, enhancing tumor cell growth and aggressiveness.\n <\/jats:p>\n <\/jats:sec>\n \n Clinical trial registration<\/jats:title>\n 2023-28.<\/jats:p>\n <\/jats:sec>","DOI":"10.1177\/03936155261421213","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T17:28:11Z","timestamp":1771522091000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["High KAP1 expression promotes pleural mesothelioma cell proliferation and metastasis"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2448-0582","authenticated-orcid":false,"given":"Wen","family":"Mei","sequence":"first","affiliation":[{"name":"The People's Hospital of Chuxiong Yi Autonomous Prefecture"},{"name":"Faculty of life Science and Technology, Kunming University of Science and Technology, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Hangzhou Medical College"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengjie","family":"Yang","sequence":"additional","affiliation":[{"name":"The People's Hospital of Chuxiong Yi Autonomous Prefecture"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qunshan","family":"Fu","sequence":"additional","affiliation":[{"name":"The People's Hospital of Chuxiong Yi Autonomous Prefecture"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Xiong","sequence":"additional","affiliation":[{"name":"Dali University"},{"name":"Dali University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yepin","family":"Zhang","sequence":"additional","affiliation":[{"name":"The People's Hospital of Chuxiong Yi Autonomous Prefecture"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCO.0000000000000697"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.jvca.2020.07.017"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-021-00954-7"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1148\/ryct.250213"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/his.15065"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2023-0304-RA"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10112434"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-021-01441-7"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.25691"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/narcan\/zcac024"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2022111179"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.201900349"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20200821"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.313262"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.4331\/wjbc.v5.i3.308"},{"key":"e_1_3_4_17_2","first-page":"258","article-title":"Expression and prognostic significance of KAP1 gene in malignant pleural mesothelioma","volume":"42","author":"Mei W","year":"2024","unstructured":"Mei W, Wang XM, Liu RA, et al. 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