{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T04:24:50Z","timestamp":1787113490580,"version":"build-2736575974"},"reference-count":45,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Yulin City Science Research and Technological Development Program","award":["202432021"],"award-info":[{"award-number":["202432021"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Biol Markers"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Lactylation has emerged as a crucial post-translational modification that influences oncogenesis and tumor progression. However, its intratumoral heterogeneity in osteosarcoma is not well characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Single-cell multi-omics data were retrieved from the Gene Expression Omnibus database. Lactylation heterogeneity was assessed, cell-cell communication networks were constructed, and transcription factor activity was analyzed to delineate differences among cell populations. Bioinformatics analysis coupled with experimental validation was employed to screen for potential targets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The study revealed that the overall lactylation level was significantly elevated in osteosarcoma tissues compared to adjacent non-tumor tissues. At the single-cell level, osteosarcoma-derived osteoblastic cells exhibited markedly higher lactylation scores than their counterparts from adjacent non-tumor tissue. Bioinformatics analysis identified NUF2 as a potential biomarker associated with the high-lactylation malignant state. Subsequent experimental validation confirmed that NUF2 is overexpressed in osteosarcoma tissues and co-localizes with pan-Kla.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study presents a single-cell lactylation atlas of osteosarcoma, identifies NUF2 as a potential biomarker associated with the high-lactylation malignant state, and provides a novel theoretical basis for the clinical translation of lactylation-targeted therapies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03936155261465683","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T09:12:56Z","timestamp":1786957976000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Single-cell multi-omics reveals NUF2 as a potential biomarker associated with the high-lactylation malignant state in osteosarcoma"],"prefix":"10.1177","volume":"41","author":[{"given":"Yan","family":"Huang","sequence":"first","affiliation":[{"name":"Yulin Orthopaedics Hospital of Integrated Traditional Chinese and Western Medicine"},{"name":"Yulin Orthopaedics Hospital of Integrated Traditional Chinese and Western Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Feng","sequence":"additional","affiliation":[{"name":"Yulin Orthopaedics Hospital of Integrated Traditional Chinese and Western Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Yang","sequence":"additional","affiliation":[{"name":"Yulin Orthopaedics Hospital of Integrated Traditional Chinese and Western Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yu","sequence":"additional","affiliation":[{"name":"Yulin Orthopaedics Hospital of Integrated Traditional Chinese and Western 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