{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T08:55:52Z","timestamp":1777712152542,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2016,1,26]],"date-time":"2016-01-26T00:00:00Z","timestamp":1453766400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunopathol Pharmacol"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p>The aim of this study is to evaluate the presence of anti-laminin-1 antibodies (aLN-1) in sera and follicular fluid (FF) of infertile women affected by Hashimoto\u2019s thyroiditis (HT) undergoing in vitro fertilization (IVF) and its impact on oocyte maturation and IVF outcome. aLN-1 were measured by a home-made enzyme linked immunosorbent assay (ELISA) in: (1) sera and FF from 44 infertile women affected by HT (HTIW) with tubal factor or male factor as primary cause of infertility; (2) in sera and FF from 28 infertile women without HT, with tubal factor or male factor as cause of infertility (infertile controls-ICTR); and (3) in sera from 50 fertile women (FW). aLN-1 serum levels were significantly higher in HTIW when compared with both fertile women and ICTR ( P &lt;0.001and P &lt;0.01, respectively). Assuming as cutoff the 99th percentile of values obtained in sera of FW, 43.2% of HTIW and 3.6% of ICTR were aLN-1 positive ( P = 0.0001). Also aLN-1 detected in FF from HTIW were significantly higher in comparison with those found in FF of ICTR ( P = 0.006). In HTIW, metaphase II oocyte count showed inverse correlation with both serum and FF aLN-1 levels (r = 0.34, P = 0.02 and r = 0.33, P = 0.03, respectively). Implantation and pregnancy rates were significantly lower in HTIW (7.9% and 9.1%, respectively) when compared with ICTR (23% and 31.1%, respectively) ( P = 0.015 and P = 0.03, respectively). Our results demonstrated for the first time the presence of aLN-1 in a relevant percentage of HTIW and suggest that these auto-antibodies may impair IVF outcome.<\/jats:p>","DOI":"10.1177\/0394632015627281","type":"journal-article","created":{"date-parts":[[2016,1,26]],"date-time":"2016-01-26T20:17:49Z","timestamp":1453839469000},"page":"280-287","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Anti-laminin-1 antibodies in serum and follicular fluid of women with Hashimoto\u2019s thyroiditis undergoing\n                    <i>in vitro<\/i>\n                    fertilization"],"prefix":"10.1177","volume":"29","author":[{"given":"Domenico","family":"Caccavo","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Human Oncology, Section of Immunology, University of Bari \u201cAldo Moro\u201d, University Hospital, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelly M","family":"Pellegrino","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Human Oncology, Section of Immunology, University of Bari \u201cAldo Moro\u201d, University Hospital, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Nardelli","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Vergine","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Leone","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Marolla","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margherita P","family":"Vacca","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaella","family":"Depalo","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Gynecology, Obstetrics and Anesthesiology, Unit of Pathophysiology of Human Reproduction and Gametes Cryopreservation, University Hospital, Bari, 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