{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T08:57:53Z","timestamp":1777712273546,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T00:00:00Z","timestamp":1507075200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunopathol Pharmacol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:p>\n                    HSP60 has been implicated in chronic inflammatory disease pathogenesis, including chronic obstructive pulmonary disease (COPD), but the mechanisms by which this chaperonin would act are poorly understood. A number of studies suggest a role for extracellular HSP60, since it can be secreted from cells and bind Toll-like receptors; however, the effects of this stimulation have never been extensively studied. We investigated the effects (pro- or anti-inflammatory) of HSP60 in human bronchial epithelial cells (16-HBE) alone and in comparison with oxidative, inflammatory, or bacterial challenges. 16-HBE cells were cultured for 1\u20134 h in the absence or presence of HSP60, H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , lipopolysaccharide (LPS), or cytomix. The cell response was evaluated by measuring the expression of IL-8 and IL-10, respectively, pro- and anti-inflammatory cytokines involved in COPD pathogenesis, as well as of pertinent TLR-4 pathway mediators. Stimulation with HSP60 up-regulated IL-8 at mRNA and protein levels and down-regulated IL-10 mRNA and protein. Likewise, CREB1 mRNA was up-regulated. H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and LPS up-regulated IL-8. Experiments with an inhibitor for p38 showed that this mitogen-activated protein kinase could be involved in the HSP60-mediated pro-inflammatory effects. HSP60 showed pro-inflammatory properties in bronchial epithelial cells mediated by activation of TLR-4-related molecules. The results should prompt further studies on more complex ex-vivo or in-vivo models with the aim to elucidate further the role of those molecules in the pathogenesis of COPD.\n                  <\/jats:p>","DOI":"10.1177\/0394632017734479","type":"journal-article","created":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T10:35:03Z","timestamp":1507113303000},"page":"333-340","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":36,"title":["HSP60 activity on human bronchial epithelial cells"],"prefix":"10.1177","volume":"30","author":[{"given":"Claudia","family":"Sangiorgi","sequence":"first","affiliation":[{"name":"Divisione di Pneumologia e Laboratorio di Citoimmunopatologia dell\u2019Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri SpA SB, IRCCS, Veruno, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Vallese","sequence":"additional","affiliation":[{"name":"Divisione di Pneumologia e Laboratorio di Citoimmunopatologia dell\u2019Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri SpA SB, IRCCS, Veruno, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella","family":"Gnemmi","sequence":"additional","affiliation":[{"name":"Divisione di Pneumologia e Laboratorio di Citoimmunopatologia dell\u2019Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri SpA SB, IRCCS, Veruno, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Bucchieri","sequence":"additional","affiliation":[{"name":"Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Universit\u00e0 degli Studi di Palermo, Palermo, Italy"},{"name":"Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Balbi","sequence":"additional","affiliation":[{"name":"Divisione di Pneumologia e Laboratorio di Citoimmunopatologia dell\u2019Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri SpA SB, IRCCS, Veruno, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Brun","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Padova, Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelo","family":"Leone","sequence":"additional","affiliation":[{"name":"Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche, Universit\u00e0 degli Studi di Palermo, Palermo, Italy"},{"name":"Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Giordano","sequence":"additional","affiliation":[{"name":"Divisione di Pneumologia e Laboratorio di Citoimmunopatologia dell\u2019Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri SpA SB, IRCCS, Veruno, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Everly","family":"Conway de Macario","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, University of Maryland \u2013 Baltimore, Baltimore, MD, USA"},{"name":"Institute of Marine and Environmental Technology (IMET), Baltimore, MD, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto JL","family":"Macario","sequence":"additional","affiliation":[{"name":"Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST), Palermo, Italy"},{"name":"Department of Microbiology and Immunology, School of Medicine, University of Maryland \u2013 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Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,10,4]]},"reference":[{"key":"bibr1-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059116"},{"key":"bibr2-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05187.x"},{"key":"bibr3-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-015-0617-9"},{"key":"bibr4-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(05)70393-4"},{"key":"bibr5-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028200"},{"key":"bibr6-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvt047"},{"key":"bibr7-0394632017734479","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0804238"},{"key":"bibr8-0394632017734479","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.10.5416"},{"key":"bibr9-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02006-2016"},{"key":"bibr10-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.10.1.7507342"},{"key":"bibr11-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"bibr12-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)02888-3"},{"key":"bibr13-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2014.04.012"},{"key":"bibr14-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2007.02788.x"},{"key":"bibr15-0394632017734479","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.02.014"},{"key":"bibr16-0394632017734479","doi-asserted-by":"crossref","first-page":"5450","DOI":"10.4049\/jimmunol.159.11.5450","volume":"159","author":"Parry 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