{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:35:24Z","timestamp":1785299724101,"version":"3.55.0"},"reference-count":24,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunopathol Pharmacol"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:p>Sepsis caused by aggressive infection is a severe clinical problem with an increasing incidence worldwide. Toll-like receptors and their common adapter myeloid differentiation factor 88 (MyD88) can activate immune responses by recognizing a foreign microbe\u2019s product. This study aimed to identify the different time expression of TLR four signaling pathway in an experimental rodent model of polymicrobial sepsis. A randomized animal study was investigated in rats with septic peritonitis induced by cecal ligation and puncture (CLP). The expressions of MyD88-dependent pathway biomarkers, including MyD88, nuclear factor-\u03baB (NF-\u03baB), and serum tumor necrosis factor-\u03b1 (TNF-\u03b1), were analyzed and compared to the sham controls at the different time points after CLP surgery. CLP-induced sepsis increased liver MyD88 mRNA expression and protein expression compared to the control groups at 2\u2009h after surgery. The MyD88 mRNA and protein expressions in rats with CLP-induced sepsis marked increased at 4 and 6\u2009h, and their NF-\u03baB activities and serum TNF-\u03b1 levels also increased at 4\u2009h after CLP surgery (both p &lt; .05). The different serial expression of MyD88-ependent pathway during sepsis may be used as biomarkers during sepsis. These results may provide further helpful information for using pro-inflammatory biomarkers of innate immunity such as MyD88 and TNF-\u03b1 in clinical sepsis or related abdominal surgical emergency in the future.<\/jats:p>","DOI":"10.1177\/03946320221090021","type":"journal-article","created":{"date-parts":[[2022,5,21]],"date-time":"2022-05-21T07:48:27Z","timestamp":1653119307000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Time-serial expression of toll-like receptor 4 signaling during polymicrobial sepsis in rats"],"prefix":"10.1177","volume":"36","author":[{"given":"En-Pei","family":"Lee","sequence":"first","affiliation":[{"name":"Division of Pediatric Critical Care Medicine, Department of Pediatrics, Chang Gung Memorial Hospital at Linko, Taiwan"},{"name":"College of Medicine, Chang Gung University, 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