{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:29:51Z","timestamp":1792852191819,"version":"4.5.0"},"reference-count":52,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Science and Technological Development Fund (STDF), Ministry of Scientific Research, Egypt","award":["15123"],"award-info":[{"award-number":["15123"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunopathol Pharmacol"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>microRNA-146a (miR-146a) plays an essential role in immune anomalies and organ injury of systemic lupus erythematosus (SLE) by regulating the disease\u2019s inflammation and complications. Here, we analyzed the expression of miR-146a in SLE and a panel of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-17, and TNF-\u03b1). Association between all measured parameters and the disease\u2019s clinical manifestation and response to treatment was monitored. Our study populations were 113 SLE patients and 104 healthy volunteers. miR-146a expression in peripheral blood mononuclear cells (PBMCs) was measured by quantitative real-time PCR (RT-qPCR). The content of the plasma cytokines (IL-1\u03b2, IL-6, IL-8, IL-17, and TNF-\u03b1) was detected by enzyme-linked immunosorbent assay (ELISA). Compared with healthy controls, miR-146a expression was significantly increased ( p &lt; 0.05) in lupus patients. The analysis of the receiver operator characteristic curve (ROC) of miR-146a showed 91% sensitivity and 70% specificity. IL-1\u03b2, IL-6, and IL-17 cytokines were significantly increased ( p &lt; 0.001), while IL-8 and TNF-\u03b1 were significantly decreased ( p &lt; 0.001) in SLE patients against controls. The expression of miR-146a and TNF-\u03b1 was upregulated considerably in SLE patients with severe disease activity. miR-146a expression was positively correlated with IL-6. Our results pointed to the elevation of miR-146a as a trade marker of SLE patients. Reduction of IL-8 and TNF-\u03b1 in combination with an elevation of IL-1\u03b2, IL-6, and IL-17 might refer to miR-146a\u2019s dual effect in controlling inflammation in lupus. Although we shed some light on the role of miR-146a in SLE, further study is recommended to improve our results.<\/jats:p>","DOI":"10.1177\/03946320231154998","type":"journal-article","created":{"date-parts":[[2023,2,5]],"date-time":"2023-02-05T21:52:05Z","timestamp":1675633925000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":20,"title":["Crosstalk between miR-146a and pro-inflammatory cytokines in patients with systemic lupus erythematosus"],"prefix":"10.1177","volume":"37","author":[{"given":"Basima A","family":"El-Akhras","sequence":"first","affiliation":[{"name":"Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1176-2727","authenticated-orcid":false,"given":"Roba M","family":"Talaat","sequence":"additional","affiliation":[{"name":"Molecular Biology 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