{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T20:04:35Z","timestamp":1788984275897,"version":"build-2803163510"},"reference-count":45,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing","doi-asserted-by":"publisher","award":["cstc2021jcyj-msxmX0197"],"award-info":[{"award-number":["cstc2021jcyj-msxmX0197"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunopathol Pharmacol"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Intestinal ischemia\/reperfusion (II\/R) injury is a life-threatening situation accompanied by severe organ injury, especially acute lung injury (ALI). A great body of evidence indicates that II\/R injury is usually associated with hyperlactatemia. G-protein-coupled receptor 81 (GPR81), a receptor of lactate, has been recognized as a regulatory factor in inflammation, but whether it was involved in II\/R injury-induced ALI is still unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To establish the II\/R injury model, the superior mesenteric artery of the mice was occluded gently by a microvascular clamp for 45\u00a0min to elicit intestinal ischemia and then a 90-min reperfusion was performed. Broncho-alveolar lavage fluid (BALF) and lung tissues were obtained to evaluate the lung injury after II\/R. The pulmonary histopathological alteration was evaluated by H&amp;E staining. The concentration of proteins, the number of infiltrated cells, and the level of IL-6 were measured in BALF. The formation of neutrophil extracellular traps (NETs) was evaluated by the level of double-stranded DNA (dsDNA) and myeloperoxidase- double-stranded DNA (MPO-dsDNA) complex in BALF, and the content of citrullinated histone H3 (Cit-H3) in lung tissue. The level of HMGB1 in the BALF and plasma was measured by enzyme linked immunosorbent assay (ELISA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Administration of the GPR81 agonist 3,5-dihydroxybenzoic acid (DHBA) aggravated II\/R injury-induced lung histological abnormalities, upregulated the concentration of proteins, the number of infiltrated cells, and the level of IL-6 in BALF. In addition, DHBA treatment increased the level of dsDNA and MPO-dsDNA complex in BALF, and promoted the elevation of Cit-H3 in lung tissue and the release of HMGB1 in BALF and plasma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>After induction of ALI by II\/R, the administration of DHBA aggravated ALI through NETs formation in the lung.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03946320231193832","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T06:37:14Z","timestamp":1694500634000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Activation of GPR81 aggravated intestinal ischemia\/reperfusion injury-induced acute lung injury via HMGB1-mediated neutrophil extracellular traps formation"],"prefix":"10.1177","volume":"37","author":[{"given":"Sun","family":"Yili","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dai","family":"Xinyi","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Kerui","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Kun","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Chongqing Medical University, Chongqing, 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