{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T08:41:03Z","timestamp":1777711263515,"version":"3.51.4"},"reference-count":48,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["NRF-2021R1A2B5B03002157"],"award-info":[{"award-number":["NRF-2021R1A2B5B03002157"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunopathol Pharmacol"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>Objectives: Our objective was to assess the effects and mechanisms of nifuratel on IgE-mediated mast cell (MC) degranulation and anaphylaxis in both in vitro and in vivo settings.<\/jats:p>\n                  <jats:p>Methods: The anti-allergic activity of nifuratel was evaluated in mast cell cultures and the passive cutaneous anaphylaxis (PCA) model. The effects of nifuratel on signaling pathways stimulated by antigen in mast cells were measured by immunoblotting, immunoprecipitation, in vitro protein tyrosine kinase assay, and other molecular biological methods.<\/jats:p>\n                  <jats:p>\n                    Results: Nifuratel reversibly inhibited antigen-induced degranulation of MCs (IC\n                    <jats:sub>50<\/jats:sub>\n                    , approximately 0.34\u00a0\u03bcM for RBL-2H3 cells; approximately 0.94\u00a0\u03bcM for BMMCs) and suppressed the secretion of inflammatory cytokines IL-4 (IC\n                    <jats:sub>50<\/jats:sub>\n                    , approximately 0.74\u00a0\u03bcM) and TNF-\u03b1 (IC\n                    <jats:sub>50<\/jats:sub>\n                    , approximately 0.48\u00a0\u03bcM). Mechanism studies showed that nifuratel inhibited the phosphorylation of Syk by antigen via the inhibition of recruitment of cytosolic Syk to the \u0263 subunit of Fc\u03b5RI, and decreased the activation of Syk downstream signaling proteins LAT, Akt, and MAPKs. Finally, nifuratel dose-dependently suppressed the IgE-mediated passive cutaneous anaphylaxis in mice (ED\n                    <jats:sub>50<\/jats:sub>\n                    , approximately 22\u00a0mg\/kg).\n                  <\/jats:p>\n                  <jats:p>Conclusion: Our findings suggest that nifuratel inhibits pathways essential for the activation of mast cells to suppress anaphylaxis, thereby indicating that the anti-microbial drug, nifuratel, could be a potential drug candidate for IgE-mediated allergic disorders.<\/jats:p>","DOI":"10.1177\/03946320231202349","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T11:47:18Z","timestamp":1694692038000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Drug repositioning of anti-microbial agent nifuratel to treat mast cell-mediated allergic responses"],"prefix":"10.1177","volume":"37","author":[{"given":"Ji Eon","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Immunology, College of Medicine, Konkuk University, Chungju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Yeong","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Immunology, College of Medicine, Konkuk University, Chungju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keun Young","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Immunology, College of Medicine, Konkuk University, Chungju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Geun","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Immunology, College of Medicine, Konkuk University, Chungju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Duksung Women\u2019s University, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyuk Soon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Natural Science and Department of Health Sciences, The Graduate School of Dong-A University, Busan, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0643-2395","authenticated-orcid":false,"given":"Wahn Soo","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Immunology, College of Medicine, Konkuk University, Chungju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,9,14]]},"reference":[{"key":"bibr1-03946320231202349","volume-title":"Type I hypersensitivity reaction","author":"Abbas M","year":"2022"},{"key":"bibr2-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.07.003"},{"key":"bibr3-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.07.040189.000423"},{"key":"bibr4-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1369\/0022155414545334"},{"key":"bibr5-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.1.123"},{"key":"bibr6-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2755"},{"key":"bibr7-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1038\/35037021"},{"key":"bibr8-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.44.27962"},{"key":"bibr9-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.08.006"},{"key":"bibr10-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(02)00396-5"},{"key":"bibr11-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.04.015"},{"key":"bibr12-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01623-10"},{"key":"bibr13-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1111\/jog.13052"},{"key":"bibr14-03946320231202349","first-page":"8","volume":"52","author":"Mendling W","year":"2002","journal-title":"Arzneimittelforschung"},{"key":"bibr15-03946320231202349","first-page":"CD013012","volume":"2018","author":"Obiero J","year":"2018","journal-title":"The Cochrane Database of Systematic Reviews"},{"key":"bibr16-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1038\/nature07204"},{"key":"bibr17-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.05.019"},{"key":"bibr18-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/507291"},{"key":"bibr19-03946320231202349","doi-asserted-by":"publisher","DOI":"10.1038\/nri1782"},{"key":"bibr20-03946320231202349","unstructured":"Pawankar R, Holgate ST, Canonica GW, et al. 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