{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:26:51Z","timestamp":1791559611067,"version":"4.3.4"},"reference-count":55,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100010613","name":"Taipei Medical University\u2013Wan Fang Hospital","doi-asserted-by":"publisher","award":["110TMU-WFH-20"],"award-info":[{"award-number":["110TMU-WFH-20"]}],"id":[{"id":"10.13039\/501100010613","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010613","name":"Taipei Medical University\u2013Wan Fang Hospital","doi-asserted-by":"publisher","award":["112-wf-eva-21"],"award-info":[{"award-number":["112-wf-eva-21"]}],"id":[{"id":"10.13039\/501100010613","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010613","name":"Taipei Medical University\u2013Wan Fang Hospital","doi-asserted-by":"publisher","award":["113TMU-WFH-18"],"award-info":[{"award-number":["113TMU-WFH-18"]}],"id":[{"id":"10.13039\/501100010613","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Higher Education Sprout Project by the Ministry of Education","award":["DP2-TMU-113-C-02"],"award-info":[{"award-number":["DP2-TMU-113-C-02"]}]},{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 113-2313-B-038-003"],"award-info":[{"award-number":["NSTC 113-2313-B-038-003"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunopathol Pharmacol"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The programmed cell death-ligand 1 (PD-L1) combined positive score is used as a patient selection tool and predictive factor for anti-programmed cell death-1 (PD-1)\/PD-L1 therapy in gastric cancer. However, the expression of PD-L1 and major histocompatibility complex class I (MHC-I) can be affected by conventional treatment approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>In this study, we examined the effects of chemotherapy on surface PD-L1 and surface MHC-I expression in living and apoptotic gastric cancer cells. AGS (moderately differentiated) and SNU-1 (poorly differentiated) cells were treated 5-Fluorouracil (5-Fu), cisplatin, mitomycin c (MMC), and FOLFOX (5-Fu, leucovorin, and oxaliplatin).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>To quantify the expression levels of surface PD-L1 or surface MHC-I on living and apoptotic cells, the cells were co-stained with annexin V and PD-L1 or MHC-I antibodies. The percentages of single positive (annexin V-negative, PD-L1-positive; annexin V-negative, MHC-I-positive) and double positive (annexin V-positive, PD-L1-positive; annexin V-positive, MHC-I-positive) cells were analyzed by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Every tested chemotherapeutic agent increased the levels of surface PD-L1 and surface MHC-I, although the extents of increase differed in AGS and SNU-1 cells. In AGS cells, 5-Fu caused the largest increases in surface PD-L1 and surface MHC-I. However, 5-Fu caused the weakest increases in surface PD-L1 and surface MHC-I in SNU-1 cells. Notably, chemotherapy-mediated increases in surface PD-L1 and surface MHC-I mostly occurred on apoptotic cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Our findings reveal that chemotherapy mainly increases surface PD-L1 and surface MHC-I on apoptotic gastric cancer cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/03946320251338662","type":"journal-article","created":{"date-parts":[[2025,5,17]],"date-time":"2025-05-17T04:06:22Z","timestamp":1747454782000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Chemotherapy elevates cell surface PD-L1 and MHC-I expression in apoptotic gastric cancer cells"],"prefix":"10.1177","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0181-0943","authenticated-orcid":false,"given":"You-Syuan","family":"Lou","sequence":"first","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8412-2434","authenticated-orcid":false,"given":"Xu-Chen","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7432-2224","authenticated-orcid":false,"given":"Chih-Cheng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Division of General Surgery, Department of Surgery, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9696-0985","authenticated-orcid":false,"given":"Yi-Hsuan","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9112-2702","authenticated-orcid":false,"given":"Tzu-Cheng","family":"Chien","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2035-8344","authenticated-orcid":false,"given":"Pei-Ling","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan"},{"name":"Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9490-4937","authenticated-orcid":false,"given":"Chu-Wan","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Nursing, National Tainan Junior College of Nursing, Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9706-8869","authenticated-orcid":false,"given":"Hsin-Hsien","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of General Surgery, Department of Surgery, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan"},{"name":"Division of General Surgery, Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0454-0624","authenticated-orcid":false,"given":"Bor-Chyuan","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,5,17]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.21037\/jgo-22-818"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.4103\/1735-5362.359430"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i2.204"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-020-01045-9"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2016.293"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2004413"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-022-01326-5"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12072636"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737140.2016.1123626"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-022-01301-0"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2022.40.4_suppl.344"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10147-019-01498-8"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10018-1321"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-024-11972-w"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12071760"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.636568"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.844866"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2024.01.009"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/4785098"},{"key":"e_1_3_3_21_2","article-title":"Chemotherapeutics and radiation stimulate MHC class I expression through elevated interferon-beta signaling in breast cancer cells","volume":"7","author":"Wan S","year":"2012","unstructured":"Wan S, Pestka S, Jubin RG, et al (2012) Chemotherapeutics and radiation stimulate MHC class I expression through elevated interferon-beta signaling in breast cancer cells. PLoS One 7: e32542.","journal-title":"PLoS One"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.05.009"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2018.08.005"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.21037\/atm-22-6049"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-23-3206"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.4678"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-022-01312-x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.134.BJR-2023-0289.R1"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15113036"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1080\/02656736.2024.2310017"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-1962-2"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34696-x"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-020-01090-4"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12193"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2022.07.004"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-023-11299-y"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1556-0864(23)00316-7"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cllc.2016.03.011"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-020-01611-6"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00932-5"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1230135"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112965"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704500103"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4780-0"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2015.12.007"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-019-02341-9"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-20-0812"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18061331"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.08.013"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2023.107438"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-024-02023-w"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/app13074340"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.07.016"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-22-0953"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-020-01734-6"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2024.216618"}],"container-title":["International Journal of Immunopathology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03946320251338662","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/03946320251338662","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/03946320251338662","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:01:08Z","timestamp":1777456868000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/03946320251338662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":55,"alternative-id":["10.1177\/03946320251338662"],"URL":"https:\/\/doi.org\/10.1177\/03946320251338662","relation":{},"ISSN":["0394-6320","2058-7384"],"issn-type":[{"value":"0394-6320","type":"print"},{"value":"2058-7384","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"article-number":"03946320251338662"}}