{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:53:47Z","timestamp":1791644027488,"version":"4.3.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2012,10,24]],"date-time":"2012-10-24T00:00:00Z","timestamp":1351036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Toxicol Ind Health"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:p>Edible mushrooms (EMs) are nutritionally rich source of proteins and essential amino acids. In the present study, the antioxidant activity via 1,1-diphenyl-2-picrylhydrazyl (DPPH) and antimicrobial potential in EMs ( Pleurotus ostreatus, Morchella esculenta, P. ostreatus (Black), P. ostreatus (Yellow) and Pleurotus sajor-caju) were investigated. The DPPH radical scavenging activity revealed that the significantly higher activity (66.47%) was observed in Morchella esculenta at a maximum concentration. Similarly, the dose-dependent concentrations (200, 400, 600, 800 and 1000\u00a0\u00b5g) were also used for other four EMs. Pleurotus ostreatus exhibited 36.13% activity, P. ostreatus (Black (B)) exhibited 30.64%, P. ostreatus (Yellow (Y)) exhibited 40.75% and Pleurotus sajor-caju exhibited 47.39% activity at higher concentrations. Furthermore, the antimicrobial potential were investigated for its toxicity against gram-negative bacterial strains ( Escherichia coli, Pseudomonas aeroginosa, Salmonella typhi, Klebsiella pneumonia, Erwinia carotovora and Agrobacterium tumifaciens), gram-positive bacterial strains ( Bacillus subtilis, Bacillus atrophaeus and Staphylococcus aureus) and a fungal strain ( Candida albicans) in comparison with standard antibiotics. Antimicrobial screening revealed that the ethanol extract of P. ostreatus was active against all microorganism tested except E. coli. Maximum zone of inhibition (13\u00a0mm) was observed against fungus and A. tumifaciens. P. sajor-caju showed best activities (12.5\u00a0mm) against B. subtilis, B. atrophaeus and K. pneumonia. P. ostreatus (Y) showed best activities against P. aeroginosa (21.83\u00a0mm), B. atrophaeus (20\u00a0mm) and C. albicans (21\u00a0mm). P. ostreatus (B) exhibited best activities against C. albicans (16\u00a0mm) and slightly lower activities against all other microbes except S. typhi. M. esculenta possess maximum activities in terms of inhibition zone against all microorganisms tested except S. typhi.<\/jats:p>","DOI":"10.1177\/0748233712463775","type":"journal-article","created":{"date-parts":[[2012,10,24]],"date-time":"2012-10-24T20:34:54Z","timestamp":1351110894000},"page":"826-834","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":24,"title":["Antioxidant activity via DPPH, gram-positive and gram-negative antimicrobial potential in edible mushrooms"],"prefix":"10.1177","volume":"30","author":[{"given":"Nisar","family":"Ahmad","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fazal","family":"Mahmood","sequence":"additional","affiliation":[{"name":"Nuclear Institute for Food and Agriculture (NIFA), Peshawar, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahid Akbar","family":"Khalil","sequence":"additional","affiliation":[{"name":"Nuclear Institute for Food and Agriculture (NIFA), Peshawar, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshan","family":"Zamir","sequence":"additional","affiliation":[{"name":"Nuclear Institute for Food and Agriculture (NIFA), Peshawar, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hina","family":"Fazal","sequence":"additional","affiliation":[{"name":"Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bilal Haider","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,10,24]]},"reference":[{"key":"bibr1-0748233712463775","first-page":"231","volume":"2","author":"Ahmad N","year":"2010","journal-title":"International Journal of Phytomedicine"},{"key":"bibr2-0748233712463775","doi-asserted-by":"publisher","DOI":"10.1007\/s11240-010-9712-x"},{"key":"bibr3-0748233712463775","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(11)60063-1"},{"key":"bibr4-0748233712463775","doi-asserted-by":"publisher","DOI":"10.1177\/0748233711407238"},{"key":"bibr5-0748233712463775","first-page":"3293","volume":"5","author":"Ahmad N","year":"2011","journal-title":"Journal of Medicinal Plants Research"},{"key":"bibr6-0748233712463775","author":"Ahmad N","year":"2012","journal-title":"Toxicology and Industrial Health"},{"key":"bibr7-0748233712463775","doi-asserted-by":"publisher","DOI":"10.5053\/ejobios.2009.3.0.8"},{"key":"bibr8-0748233712463775","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-006-0394-x"},{"key":"bibr9-0748233712463775","unstructured":"Christensen CM (1985). 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